脱落酸和水杨酸对9号淡水小球藻生长及总脂含量的影响研究毕业论文.doc

SHANDONGＵＮＩＶＥＲＳＩＴＹ　ＯＦ　ＴＥＣＨＮＯＬＯＧＹ 毕业论文 脱落酸和水杨酸对9号淡水小球藻生长及总脂含量的影响研究 学 院： 专 业： 学生姓名： 学 号： 指导教师： 年 月 摘要 微藻中的油脂是一种很有前途的用于生产生物柴油的原料。外源激素对9号淡水小球藻的生长及油脂积累的影响已有所研究。本文通过向9号小球藻细胞培养液中添加不同量的激素，研究不同浓度的激素对9号小球藻细胞生长及油脂积累的调控作用。脱落酸组与水杨酸组分别设置了6个不同浓度的量，其中各有一个空白对照组。实验结果表明，9号小球藻细胞在激素浓度为500μg/L时生长最好，生物量最大。高浓度的激素促进细胞中油脂的合成与积累，50mg/L浓度的脱落酸最能促进9号小球藻细胞中油脂的合成与积累，且单位油脂含量最高，是对照组的1.8倍；20mg/L浓度的水杨酸最能促进小球藻细胞中油脂的合成与积累，且单位油脂含量最高，是对照组的1.6倍。但是高浓度的激素抑制9号小球藻的生长，微藻细胞的高生长率与高油脂含量是相矛盾的，他们很难同时兼备。 关键词：9号淡水小球藻，脱落酸，水杨酸，生长特性，油脂积累 Abstract Microalgae lipid is a promising feedstock for biodiesel production. Effect of Exogenous hormones on the growth and accumulation properties of microalgae Chlorella 9 was studied. This thesis have studied the regulation effects on microalgae Chlorella 9, which was added different amounts of hormones by the culture liquid. Abscisic acid and salicylic acid group, respectively, set up the quantity of 6 different concentrations, which each have a blank control group. The experimental results show that the microalgae Chlorella 9 cells in hormone concentration for 500 μg/L grow best when the biomass maximum. High concentrations of the hormone can promote the lipid synthesis and accumulation in the microalgae cells, 50mg/L abscisic acid can promote the most microalgae Chlorella 9 cells in the synthesis and accumulation of liquid, the liquid per content is highest and 1.8 times that of the control group; 20mg/L salicylic acid contributes most to the microalgae Chlorella 9 cells synthesis and accumulation of liquid, and the liquid per content is highest, 1.6 times that of the control group. However, high concentrations of hormone suppression on the microalgae Chlorella 9 cells growth, the high growth rate and high lipid content of microalgae are in contradiction, they can hardly be both high simultaneously. Key words: microalgae Chlorella 9, abscisic acid, salicylic acid, growt