艾纳香种苗快繁技术研究(唐松1稿).doc

艾纳香种苗快繁技术研究 唐松1 黄燕芬1,2 孔丽1 罗查梅1 马俊飞1 文应劲1 (1贵州师范学院化学与生命科学学院，贵州贵阳 550018；2贵州省生物资源开发利 用特色重点实验室，贵州贵阳 550018) 摘 要 本试验以大艾品种艾纳香的幼嫩茎段为外植体进行组培快繁技术研究，通过试验，优化艾纳香芽诱导、增殖及生根的不同培养基配方及组培苗移栽对环境条件的要求，从而提高外植体的诱导率，无菌苗的增殖率，组培苗的移栽成活率，为艾纳香的种苗快繁技术提供参考。结果表明：①在一定范围内，芽增殖率随6-BA浓度的增加而增大，但浓度过高则诱导效果减弱。最佳增殖初代培养基为MS+6-BA2.0 mg/L；②最佳增殖继代培养基为MS+6-BA 2.5 mg/L+NAA0.3 mg/L；③最佳的生根培养基为1/2 MS+NAA0.5mg/L；④经过炼苗的植株成活率比直接移栽的成活率要高；移栽于泥炭土与珍珠岩比为3︰1的混合营养杯中比移栽于腐殖土中的成活率高，可达92.16％。 关键词 艾纳香；组织培养；快速繁殖 Study on Rapid Propagation Technology of Blumea balsamifera seedlings HUANG Yan-fen1 TANG Song1,2 KONG Li1,2 LUO Cha-mei1,2 MA Jun-fei1,2 WEN Ying-jing1,2 (1 College of chemistry and life science, Guizhou Normal College, Guizhou Guiyang 550018; 2 Guizhou Province Key Laboratory of biological resources development and utilization characteristics, Guizhou Guiyang 550018) Abstract The test with the tender stem segments AI large varieties of Blumea balsamifera were used as explants for Study on tissue culture and rapid propagation , Through the study ,Optimization of different medium formula of Blumea balsamifera bud induction, proliferation and rooting and Requirements of external conditions of tissue culture seedling transplanting, to improve the induction rate of explant, aseptic seedling proliferation rate, to provide a technical reference for the quick propagation of Blumea balsamifera. The results showed that: ①In a certain range, bud proliferation rate increased with increasing of 6-BA concentration, but excessive concentrations induced effect weakening; ②Effects of 6-BA and NAA on proliferation of axillary buds, the best subculture medium was MS+6-BA2.5 作者简介：唐松，贵州师范学院生物资源科学专业在读本科生。 通讯作者：黄燕芬，教授，从事植物资源开发利用技术研究，E-mail：gyhyf@126.com 。 资助项目：1.教育部教改项目：生物资源科学专业综合改革试点（2012287）；2.贵州师范学院学生科研项目：艾纳香种苗快繁技术研究 mg/L+NAA0.3 mg/L; ③Effect of NAA on Seedling Roots, The best medium for rooting was 1/2 MS+NAA0.5mg/L; ④After the seedling survival rate of plant than the direct transplanting