木质素论文.doc

枣果实内果皮发育过程中木质素沉积动态分析 学生姓名：刘旭洋 导师姓名：王晓琴 摘要 本次实验是以无核金丝小枣作为实验材料，以有核金丝小枣作为对照，对果实内果皮部分各发育时期石蜡切片进行观察，以及枣的各项生理指标，初步探讨枣果实出现无核形状的机理。所得研究结果如下： 1、无核金丝小枣的发育根据石蜡切片和生理指标的特征可以分为四个时期：果实缓慢生长期（花后15天内）、果实快速生长期（花后15-30天）、果核形成期（花后30-45天）、果肉快速生长期（花后45天-成熟时） 2、无核金丝小枣的含水量要比有核金丝小枣的含水量高，证明无核金丝小枣的口感更佳。 3、果实成熟时，在内果皮部分石细胞化程度上，有核金丝小枣要比无核金丝小枣的高出许多。 关键词 枣；木质素；内果皮；观察；石蜡切片 Analysis of lignin deposition in jujube fruit peel during fruit development Name of student:Liu Xu-Yang Name of tutor:Wang Xiao-Qin Abstract In this study, we analyzed the changed of lignin deposition in jujube fruit peel during fruit development using gold thread jujube with nuclear or without nuclear. We also analyzed the fruit growth and their water content. The results were as follow: 1. The fruit development was divided into four stages: slow growth (15 days after flowering); fast growth (15-30 days after flowering); nucleation (30-45 days after flowering); pulp fast growth (45 days after flowering - maturity). 2. The water content of gold thread jujube without nuclear was higher than that of gold thread jujube with nuclear. Therefore, the fruit of gold thread jujube without nuclear might taste better than that of gold thread jujube with nuclear. 3. When fruits ripen, stone cell distribution in endocarps of gold thread jujube with nuclear was much more than that of gold thread jujube without nuclear Key words Jujube; lignin; endocarp; Observation; Paraffin section 摘要 Abstract 目 录 1.引言 1 2.材料与方法 3 2.1材料 3 2.2实验方法 3 2.2.1果实的生长曲线 3 2.2.2果实石蜡切片 3 2.2.2.1固定 3 2.2.2.2冲洗 4 2.2.2.3脱水 4 2.2.2.4浸蜡 4 2.2.2.5包埋 4 2.2.2.6切片 4 2.2.2.6染色 4 2.2.3内果皮区域的木质素含量测定 5 2.2.3.1样品剥离 5 2.2.3.2浓硫酸反应 5 2.2.3.3高压灭菌 5 2.2.3.4抽滤 6 2.2.3.5称量 6 3.结果与分析 6 3.1果实的生长情况观察 6 3.1.1生长曲线 6 3.1.2每日增长量 7 3.1.3干湿重 8 3.1.4含水量 8 3.2石蜡切片观察 9 3.3木质素含量变化 9 4.结论与讨论 10 4.1果实生长情况 10 4.2石蜡切片的困难 11 5.结论 12 参考文献 13 致谢 15 1.引言 枣，学名?Ziziphus jujuba Mill.?（《Flora of China》）材料与方法 μm。用右手转动切片机手轮，协调地进行切片操作。切下的蜡带，一端用镊子轻轻拉起，应尽可