各种缓冲液的制方法.doc

1． 甘氨酸–盐酸缓冲液（0.05mol/L）6 Q) w# m3 E4 ?+ V2 ^1 C, jX毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀释至200毫升! d: c G5 c0 u- x ppH% a( Z9 p$ ?5 e O* T! ?5 t# H hXY+ r. V1 V. L, ]: V3 H% QpHX5 E Y- x( x3 ?Y/ y9 m, t4 p4 B, J( H! K8 N2.02.42.62.89 p: ]6 z* A+ p4 B: S5050, v. T+ a$ Z$ u, Z ]0 K+ [50/ a/ W4 v$ m* t7 B/ n$ R. z; d5044.0! |9 u# K+ k# v q1 K- T% q32.4% n* A# H3 [ f) x5 y24.216.8. R i: g5 U [3.0: Q. }6 C$ T( }$ N9 C: y3.23.4$ k. X- d- C4 V) m# X) y1 A. K3.61 X5 N L: _; R @) u, ?1 T, L: q( q50% N) N1 I# n1 L1 X# l5050 {, H h I0 c5011.4/ H ~7 n7 J: l9 Y8.26.4( a7 T H! w6 W0 F/ R) d5.0甘氨酸分子量 = 75.07，0.2 mol/L甘氨酸溶液含15.01克/升。1 l8 h4 x ^; {4 |, ~7 n; e- ^7 k* c C C ~/ T2．邻苯二甲酸–盐酸缓冲液（0.05 mol/L） c: B+ s0 b/ D* Q( RX毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCl，再加水稀释到20毫升2 U/ h; ?; ~ w9 R3 {! _8 s2 f3 O2 WpH(20℃)* o+ L7 ]5 E$ Y7 C% y) f) d6 g+ HXY$ Z2 v. w9 w% P- w! UpH(20℃)X% S2 S* v0 g) ?* F% xY2.22.4! q: s4 v: o o/ C2.62.8, E( N3 ~* C8 x, P/ O: Y3.055; j, k: \2 g3 t% n8 a1 b: A5% s( q5 R# o7 H+ @; K550 {6 M/ J, i9 V4.070 g. y# {# a ~5 z [; ~3.960: t# d L7 `1 W5 \ u9 i G3.2952.6422.0226 R4 ?4 I: s5 h2 ~3.23.47 w/ w: D$ P( L3.6, M1 f, m: g, N3 C6 e. q3.855 _% h8 e A) R. A54 r, ]1 [8 Q2 f W6 w* B5 x5- N v+ A$ }; C2 u ~1.4700.990# H7 x X \2 W0.597+ n) _6 V2 m3 R B o- Y% j0.263( O9 t( H( g) W邻苯二甲酸氢钾分子量 = 204.23，0.2 mol/L邻苯二甲酸氢溶液含40.85克/升; C3 _8 O ] I% g) `0 F5 o8 {/ y. f( @7 t K W) k3．磷酸氢二钠–柠檬酸缓冲液( }5 R ^0 o7 G; I7 B% UpH0.2mol/L Na2HPO4（毫升）0.1mol/L H6 Z6 z; \! v柠檬酸（毫升）pH2 n( ?5 D! v e0.2mol/L Na2HPO4* O: ~9 m) E- t7 t \+ u: A N（毫升）0.1mol/L柠檬酸 ^4 i1 V7 J6 Z) i; d（毫升）, X. _8 ]3 ^ \$ t+ }2.2, c6 A: g4 U% [4 d1 N2.42.68 F9 C3 R# i8 m$ @$ }9 G; a2.83.03.23.43.64 O V( V S$ x: l4 `) {3.84.06 b# Y8 _! I7 h( p( f6 a, C8 j6 R4.22 Z- c0 @ _7 j3 U6 S( L4.44.64.8, P$ X9 T X u/ Y* w5.06 P% ^( K2 N$ N6 o0 C8 d; K P: k0.40 m4 _* k! T) w1.242.183.174.114.945.70: T/ j+ [0 L) x6.447.10/ Z. {0 w6 M) z O7.718.288.829.