纳米技术在微生物研究和检验中的应用_培训课件.ppt

Figure 2. Amplified anthrax bar-code DNA detection with the Verigene ID system. (A) Anthrax bar-code DNA detection with 30 nm NP probes. (B) Quantitative data of spot intensities with 30 nm NP probes (Adobe Photoshop, Adobe Systems, Inc., San Jose, CA). The horizontal line represents control signal intensity (47 ( 2). Figure 3. Single base mismatch experiment. Analytical Chemistry, 2005 , 77:24 利用生物条码技术检测核酸方法 Figure 1. Fluorescence measurements of Alexa-488-labeled barcode DNA liberated from 30- (a) and 13-nm (b) Au-NPs after assay completion. The dashed lines are five standard deviations above the negative control. 寡核苷酸金粒子探针检测DNA 纳米金探针检测核酸方法 纳米金探针检测DNA阵列的电检测 荧光染料标记寡核苷酸探针，通过荧光的光谱就能显示目标序列 纳米DNA探针检测核酸方法 谢谢！ * Nam等建立了一种超灵敏的检测蛋白质的方法，在这个系统中使用了磁性微球（前列腺特异性抗原PSA单抗标记）和联有特异性DNA片段的纳米粒子（同时PSA单抗标记）两种试剂，通过夹心法与血清中的PSA形成复合物，在磁场下分离血清中的PSA,然后用PCR或基因芯片技术检测，其灵敏度比常规的临床检测方法高六个数量极［6］。 In a typical PSA-detection experiment, an aqueous dispersion of MMP probes functionalized with mAbs to PSA (50 l of 3 mg/ml magnetic probe solution) was mixed with an aqueous solution of free PSA (10 l of PSA) and stirred at 37°C for30 min (Step 1). A 1.5-ml tube containing the assay solution was placed in a BioMag microcentrifuge tube separator (Polysciences, Incorporated, Warrington, PA) at room temperature. After 15 s, the MMP-PSA hybrids were concentrated on the wall of the tube. The supernatant (solution of unbound PSA molecules) was removed, and the MMPs were resuspended in 50 l of 0.1 M phosphate-buffered saline (PBS) (repeated twice). The NP probes (for 13-nm NP probes, 50 l at 1 nM; for30-nm NP probes, 50 l at 200 pM), functionalized with polyclonal Abs to PSA and hybridized bar-code DNA strands, were then added to the assay solution. The NPs reacted with the PSA immobilized on the MMPs and provided DNA strands for signal amplification and protein identification (Step 2). This solution was vigorously stirred at 37°C for 30 min. The MMPs were then washed with 0.1 M PBS wit