组织胚胎学实验讲义.ppt

Zhong jie Li（李仲杰） School of Medicine,Zhejiang University Email：lizhongjie@ Final score of Histology Embryology???????????  1) Attendance and Picture drawing : 5% 2) Quiz: 10% (each quiz 5%)3) Lab test: 25% 4) Final written exam: 60% Preparation of laboratory work Have the tools ready: Paper Pencils (red-blue pencil) rubber, ruler and so on Contents Requirements A. Be familiar with the structure of the light microscope B. Master the use of the light microscope. C. Understand the procedures of paraffin sectioning and H E staining. The structure of the microscope Mechanical part Optical components Total magnification Normal ocular lenes: 10× Normal Objective lenes: 4× ,10× ,40× oil immersion objective: 100× Total magnification is obtained by multiplying the magnifying power of the objective and ocular lenes. Procedure of observation First ,observe the contour, morphology and the staining of the specimen with naked eyes. Second, observe the general structure and its components with low power objective. Third , then select a representative area and observe it in detail with high power objective, then with the oil immersion lens when necessary. Make a drawing of the representative area and labeled it clearly. Interpret your sample Must think in 3 dimensions Structure and function part and whole. Horizontal section longitudinal section oblique section Simple columnar epithelium different azimuth slice map Preparing a Biological Specimen for Light Microscopy Stretched Ground section Smear The procedures of paraffin sectioning and H E staining. 1. Obtaining 2. Fixation 3.Dehydration 4. Clearing 5. Embedding 6. Sectioning 7. Staining 8. Mounting Stain methods H.E hematoxylin苏木精 eosin伊 红 Acidic stucture： Nucleus RER Free ribosome Basic stucture： Cytoplasma lysosome、Mit.,fibers HE The request for drawing Should be observe the slide carefully The request for drawing: to be