免疫亲和色谱柱制备方法.docx

Instructions 71-7086-00 AF Affinity mediaGE Healthcare CNBr-activated Sepharose? 4B CNBr-activated Sepharose 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.通用电气医疗集团CNBr-activated琼脂糖?4 b CNBr-activated琼脂糖4 b是固定地激活介质的包含一级胺配体。它提供了一个非常方便的方法固定溴化氰配体的方法。偶联反应是自发的,快速和容易执行。应用领域涵盖固定的蛋白质、多肽和核酸。Table 1. Medium characteristics.Bead structure: 4% agaroseSpacer: NoneCoupling capacity: 25–60 mg α-chymotrypsinogen/ml drained mediumBead size range: 45–165 μmAverage bead size: 90 μmMax linear flow rate*: 75 cm/h at 25°C, HR 16/10 column, 5 cm bed heightpH stability**:表1。媒介的特征。琼脂糖珠结构:4%间隔:没有耦合能力:α-chymotrypsinogen外地毫克/毫升排水媒介珠大小范围:45 - 165μm平均珠大小:90μm最大线性流量*:75厘米/小时25°C,人力资源列16/10,5厘米床层高度pH值稳定* *:Long term: 3–11Short term: 2–11Chemical stability***: Stable to all commonly used aqueous solutions.Can be used with non-ionic detergents, denaturingsolvents, e.g. 8 M urea and 6 M guanidine hydrochloride. Stable in organic solvents, such as 50% dimethylformamide and 50% dioxane.Autoclavable: Not recommended长期:3-11短期:2 - 11化学稳定性* * *:所有常用的水稳定的解决方案。可以用于非离子去污剂,变性溶剂,例如8 M尿素和6 M胍盐酸。等有机溶剂、稳定50%二甲基甲酰胺和二氧六环的50%。Autoclavable:不推荐* Linear flw rate = volumetric flw rate (cm 3/h) /column cross-sectional area (cm 2)** The ranges given are estimates based on our knowledge and experience. Please note the following:pH stability, long term refers to the pH interval where the gel is stable over a long period of time without adverse effects on its subsequent chromatographic performance. *线性flw率=容积flw率(厘米3 /小时)列横截面积(2厘米)* *给出的范围的估计是基于我们的知识和经验。请请注意以下几点:pH值稳定、长期的pH值间隔指的是凝胶稳定在很长一段时间没有不利影响在其随后的色谱性能。pH stability, short term refers to the pH interval for regeneration, cleaning-in-place and sanitization procedures.*** Data refer to the coupled produc