实验性猕猴2型糖尿病模型的建立及评价.doc

实验性猕猴2型糖尿病模型的建立及评价 宋巧巧1，周慧良2，镇海涛1，王 娜1，邓 晶1，王金祥3，潘兴华3 (1湖北科技学院内科教研室，湖北省咸宁市 437100；2咸宁市中心医院，湖北省咸宁市 437100；3解放军昆明总医院干细胞与组织器官工程研究中心，云南省干细胞工程实验室，云南省昆明市 650032) 引用本文：宋巧巧，周慧良，镇海涛，王娜，邓晶，王金祥，潘兴华. 实验性猕猴2型糖尿病模型的建立及评价[J].中国组织工程研究，2016，20(40):6048-6053. DOI: 10.3969/j.issn.2095-4344.2016.40.018 ORCID: 0000-0003-2669-2546(宋巧巧) 文章快速阅读： 文题释义： 2型糖尿病动物模型：关于2型糖尿病动物模型的研究，国内外均主要集中于兔、大鼠、小鼠，其方法涉及自发性动物模型、转基因、高脂饲料诱导和化学诱导等，这些动物模型对2型糖尿病的基础研究和防治具有重要意义，但这些非灵长类种属的动物模型与人类糖尿病的发病改变存在一定差异。非人灵长类动物和人类更为接近，其模型能更好地模拟人类糖尿病病理生理及发病过程，可靠性更高。目前国内外关于2型糖尿病非人灵长类动物模型的研究报道很少，尚缺乏标准化制作方法和评价标准。 链脲佐菌素诱导糖尿病模型：链脲佐菌素可选择性破坏一定种属动物的胰岛β细胞，能诱发许多动物产生糖尿病，采用一次大剂量静脉注射链脲佐菌素，可导致大部分胰岛β细胞出现Establishment and evaluation of a rhesus monkey model of experimental type 2 diabetes mellitus Song Qiao-qiao1, Zhou Hui-liang2, Zhen Hai-tao1, Wang Na1, Deng Jing1, Wang Jin-xiang3, Pan Xing-hua3 (1Department of Internal Medicine, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China; 2Xianning Central Hospital, Xianning 437100, Hubei Province, China; 3Stem Cell Engineering Laboratory of Yunnan Province, Stem Cells and Organ Tissue Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, Yunnan Province, China) Abstract BACKGROUND: At present, there are few reports about the non-human primate models of type 2 diabetes mellitus in domestic and abroad, so it lacks of standardized production methods and evaluation criteria. OBJECTIVE: To establish a safe and effective type 2 diabetes mellitus model of rhesus monkey and evaluation method. METHODS: Twelve rhesus monkeys were randomly assigned to experimental group (n=9) and control group (n=3). Rhesus monkeys in the experimental group were fed with high-glucose and high-fat diet for 4 weeks, and intraperitoneally injected with 30 mg/kg streptozotocin to establish models of type 2 diabetes mellitus. Rhesus monkeys in the control group were fed with an equal volume of physiological saline. At 12