配位化学在细胞凋亡中的应用课件.pptVIP

配位化学在细胞凋亡中的应用 报告人：张煜 导师：唐瑜教授 配位化学在细胞凋亡中的应用 配位化学在细胞凋亡中的应用 凋亡细胞的特征 凋亡细胞的特征 配位化学在细胞凋亡中的应用 第二步、基于MTT比色法检测IC50值的实验 Cells were exposed to each compound for a total of 48 h. Using the colorimetric mitochondrial function-based MTT viability assay, the IC50 values (final concentration≤0.5% DMSO) were calculated from dose-response curves obtained by nonlinear regression analysis. IC50 values are concentrations of drug required to inhibit tumor cell proliferation by 50%, compared to the control viability.As demonstrated by the IC50 for 48 h values listed in Table 4, the tested ferrocene bioconjugates do not show significant antiproliferative activity but the gold complexes appeared to be quite effective as cytotoxic agents against in vitro growth of various cancer cell lines. MTT比色法原理 IC50值的简介 IC50是指被抑制一半时抑制剂的浓度，这里的反应可以是酶催化反应，抗原抗体反应等。在凋亡方面，可以理解为一定浓度的某种药物诱导肿瘤细胞凋亡50%，该浓度称为50%抑制浓度，即凋亡细胞与全部细胞数之比等于50%时所对应的浓度，IC50值可以用来衡量药物诱导凋亡的能力，即诱导能力越强，该数值越低，当然也可以反向说明某种细胞对药物的耐受程度。 IC50 (48 h) of complexes against HeLa, MCF-7, and N1E-115 cells. 第三步、流式细胞记数法 Flow cytometry studies using double staining with Hoechst 33342 and propidium iodide showed also a significative percentage of apoptotic cells after incubation of MCF-7 cells with increasing concentrations of compound 10 (10 and 50 μM) for 3 and 24 h (Fig. 4 and Table 5). The use of the molecular probe H2DCF-DA showed that incubation of MCF-7 cells with compound 10 produced an increasing dose and time dependent amount of reactive oxygen species (ROS) (Fig. 5 and Table 6). Table 5Percentage of apoptotic, dead and live cells after incubation with complex 10. Table 6Production of reactive oxygen species (ROS) by MCF-7 cells incubated with complex 10. 三、设计思路 Content 四、小结 Thank you! * * * * 一、细胞凋亡的简介 二、文献 三、设计思路 四、总结 Text Text Text 一、细胞凋亡的简介 1972年Kerr根据细胞发生与坏死不同的死亡现象，提出了细胞凋亡的概念，称为Apoptosis，宣告了对细胞凋亡的真正探索的开始。而细胞程序性死亡（programmed cell death，PCD）最初是1956年发育生物学中提出的概念，是个功能性概念，强调的是其分子生物学和生理功能，一般指生理性细胞死亡。描述在一个多细胞生物体中某些细胞死亡是个体发育中