银屑病患者体外T淋巴细胞对白介素8的化学趋化功能.x.docx

银屑病患者体外T淋巴细胞对白介素8的化学趋化功能银屑病患者体外T淋巴细胞对白介素8的化学趋化功能中华皮肤科杂志 1998年第2期第31卷 论著作者：郑敏　孙国均　蔡绥勍单位：310009 杭州，浙江医科大学附属第二医院皮肤科(郑敏　孙国均　蔡绥?)；德国基尔大学皮肤病医院(Ulrich Mrowietz)关键词：银屑病；皮炎；特应性；趋化性；白细胞；白细胞介素8【摘要】　目的　探讨银屑病外周血循环T细胞体外化学趋化功能及其在银屑病发病机理中的作用。方法　运用48孔微型化学趋化装置检测了45例寻常型银屑病患者、21例异位性皮炎患者和20例正常人的纯化T细胞对重组人白介素8(IL-8)的化学趋化反应，同时用单克隆抗体荧光免疫法在流式细胞仪上测定患者和正常人的纯化T细胞表面的HLA-DR和IL-2R等激活表型以及用ELISA测定患者和正常人血清中IL-8的水平。结果　①严重银屑病和异位性皮炎患者组的循环T细胞对IL-8的体外化学趋化反应明显低于正常人对照组，而轻型银屑病患者组的循环T细胞与正常人无异；②严重银屑病、异位性皮炎组患者T细胞表面HLA-DR及IL-2R激活表型表达较正常人对照组显著增加；③银屑病、异位性皮炎患者血清IL-8水平明显高于正常人对照组。结论　银屑病患者循环T细胞在体外对IL-8的化学趋化功能明显受损，其原因可能与患者体内T细胞过度激活以及血清IL-8水平过高有关。T-lymphocyte Chemotaxis to IL-8 in vitro in Patients with PsoriasisZheng Min, Sun Guojun, Cai Suiqing, et al. Department of Dermatology, The Second Affiliated Hospital of Zhejiang Medical University, Hangzhou 310009【Abstract】　Objective　To study the chemotactic activity of T-lymphocytes from patients with psoriasis to proinflammatory cytokines in vitro and its role in the pathogenesis in psoriasis.Methods　A 48 microchemotaxis chamber was employed to determine T-cell chemotactic activity. In addition, the expression of T cell activation markers such as HLA-DR and interleukin 2 receptor was analysed with fluorescence activated cell sorting technique and serum IL-8 level was measured with ELISA. 45 patients with psoriasis (23 patients with severe psoriasis and 22 patients with mild psoriasis) and 21 patients with atopic dermatitis were investigated, and 20 healthy controls were tested equally.Results　①T-cell chemotactic responses were significantly decreased in patients with severe psoriasis and atopic dermatitis as compared to healthy controls. ②Increased expression of activation markers such as HLA-DR and interleukin 2 receptor was demonstrated in circulating T cells from severe psoriatic patients and atopic dermatitis patients in comparison to healthy controls. ③Serum IL-8 level was significantly increased in patients with psoriasis and atopic dermatitis.Conclusion　The in vitro