不同培养基分离培养胎鼠肺血管内皮细胞-第三军医大学学报.docVIP

新生小鼠肺微血管内皮细胞的培养和抗原制备 赵军1,2 路静1 赵继敏1 杨洪艳1 翟景明1 李珊1 张曦3 董子明1＊ (1郑州大学基础医学院病理生理学教研室 河南 郑州450052 2长治医学院附属和济医院呼吸科 山西 长治 046000 3 郑州大学第三附属医院妇产科Culture of lung microvascular endothelial cells in neogenetic mice and preparation of the antigen Zhaojun1,2, Lujing1, Yang Hongyan1,Zhao Jimin1, Zhai Jingming1,Lishan1, Zhangxi,Dong Ziming1＊（1 Department of Pathophysiology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, China,2 Department of respiratory, Heji hospital affiliated Changzhi medical college,changzhi 046011 China, 3 Department of Gynecology and Obstetrics,the Third Affiliated Hospital, Zhengzhou 基金项目：河南省卫生厅科技攻关项河南省教育厅自然科学研究计划项目 2008A3310018 Supported by the Scientific technique of Public Health Department of Henan Province (2008A3310018) and the Natural Science Foundation of the Education Department of Henan Province(2008A3310018) 作者简介：赵军，女，山西省长治市人，汉族，博士研究生，主治医师，主攻肺癌的生物治疗，发表论文15篇。TelEmail：zhaojun_2@。dongzm@ 收稿日期： 2008-03-31 修回日期： University, Zhengzhou 450052, China) Abstract Objective To construct a simple and effective method for the isolation and primary culture of pulmonary microvascular endothelial cells (PMVECs) in neogenetic mice and preparation of the antigen. Methods　The lung tissue of neogenetic BALB/C mice was cut into blocks and were primary cultured. The cell morphology was observed using invertedmicroscope and inverted phase contrast microscope and identified using immunohistochemical methods. Then, the cultured PMVECs were prepared to antigen through freeze and thaw, boiled and Ultrasonic disruption methods.Results　The cultured PMVECs of neogenetic BALB/C mice in vitro showed fusiform shape orpolygon, and the monolayer cultures displayed a typical paving stone morphology and capillary tube～like formationltrasonic disruption method was better than the other two methods.Conclusion　The pure PMVECs of mice can be attained rapidly and easily by the described method above. Preparation of the