绿海龟健康幼体心肝肾功能血液生化指标参考值研究.docx

绿海龟健康幼体心肝肾功能血液生化指标参考值研究成奇,潘连德（上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室，上海 201306）摘要：采集了39只人工养殖的绿海龟健康幼体(1.5~2.5龄)、6只绿海龟健康成体(10~20龄)和3只绿海龟野生成体，用Beckman AU5800和TNI BECKMAN Access 2测定和分析了反映绿海龟心肝肾功能的17项血液生化指标，结果发现野生个体的生化指标与人工养殖的绿海龟差异较大，谷草转氨酶(P=0.002)、白蛋白(P=0.015)、碱性磷酸酶(P=0.010)、谷丙转氨酶(P=0.010)等均有明显差异，人工养殖绿海龟除碱性磷酸酶与年龄呈负相关外没有明显差异，不同性别的个体在生化指标上差异不显著。本试验结果与野生绿海龟各研究结果对比发现，野生个体的指标差异非常显著，难以作为健康指标的参考。本研究建立了人工绿海龟的心肝肾功能血液生化指标参考值，为野生绿海龟血液生化研究提供参考，为指导绿海龟养殖过程中的疾病早期诊断和预防提供依据。关键词：绿海龟；人工养殖；健康幼体；血液生化；参考值中图分类号：S852.5+3DOI:10.12024/jsou.20151101601Research on the plasma biochemistry of cardiac function, liver function and renal function from healthy larva green turtles (Cheloniamydas) in captivityCHENG Qi, PAN Liande*（Key Lab of Exploration and Utilization of Aqustic Resources,Ministry of Education, Shanghai Ocean University， Shanghai 201306，China)Abstract:The paper took 17 biochemistry items of the 39 healthy captive hatching larvae green turtles (1.5-2.5 years old), six healthy adult green turtles (10-20 years old) and 3 wild turtles using the Beckman AU5800 and TNI BECKMAN Access 2 to analyze. The the result showed that there was large difference between wild and captive ones on aspartate aminotransferase (P=0.002), albumin (P=0.015), alkaline phosphatase (P=0.010), glutamic pyruvic transaminase (P=0.010) etc. The biochemistry indexes appeared no difference in captive green turtles between different age and different sex except for alkaline phosphatase. Alkaline phosphatase and age showed a negative correlation. Compared with the research results of others’ and the experiment’s wild green turtles, the indexes of wild individuals had gient difference which could not consider to be the reference for health indicators. This study established blood biochemistry reference values of captive green turtles in cardiac, liver and renal function. This provide a reference for the wild green turtles’ blood biochemistry research and establish a basis for the early diagnosis anddisease prevention of captive gree