原花青素对人冠状动脉平滑肌细胞增殖和凋亡的影响原花青素对人冠状动脉平滑肌细胞增殖和凋亡的影响.doc

原花青素对人冠状动脉平滑肌细胞增殖和凋亡的影响 罗小平，杨进，程宇，徐朝军 作者单位:415000湖南省常德卷烟厂职工医院心内科(罗小 平);410007长沙市，湖南中医药大学第一附属医院胸心外科(杨进、 程宇、徐朝军) 【摘要】目的研究原花青素对人冠状动脉平滑肌细胞增殖和凋亡的影响。方法采用MTT实验检测原花 青素对体外培养的人冠状动脉平滑肌细胞增殖的影响，运用流式细胞术、基因组DNA电泳观察凋亡特征性“梯状”条 带检测细胞凋亡，采用Caspase活性定量检测试剂盒分析Caspase-9、Caspase-3的活性。结果原花青素对人冠状动脉 平滑肌细胞具有明显的抑制作用，且其作用有剂量依赖性。流式细胞仪检测显示原花青素可以显著诱导人冠状动脉 平滑肌细胞凋亡，DNA电泳显示原花青素作用于人冠状动脉平滑肌细胞后出现凋亡细胞特有的DNA阶梯状条带。 原花青素作用后人冠状动脉平滑肌细胞Caspase-9、Caspase-3活性明显升高。结论原花青素可能通过线粒体信号通 路抑制人冠状动脉平滑肌细胞的生长并诱导其凋亡。 【关键词】原花青素;人冠状动脉平滑肌细胞;细胞凋亡 【中图分类号】R 543．3【文献标识码】A【文章编号】1674－3296(2010)20－0020－03 Effect of procyanidin on proliferation and apoptosis in human coronary artery smooth muscle cells LUO Xiao-ping * ， YANG Jin，CHENG Yu，et al．*Department of Cardiovasology，The Staff-worker Hospital of Changde Cigarette Factory，Hunan 415000，China 【Abstract】Objective To study the effect of procyanidins on proliferation and apoptosis in human coronary artery smooth muscle cells．Methods The effect of procyanidin on cell viability in human coronary artery smooth muscle cells was e- valuated by MTT assay．Cell apoptosis was observed by flow cytometry and DNA ladder analysis．Activities of Caspase-9 and Caspase-3 were assayed with Caspase colorimetric assay kit．Results Procyanidin on human coronary artery smooth muscle cells was significantly inhibited，and their effects were dose dependent．Flow cytometry showed that procyanidin can significantly induce apoptosis in human coronary artery smooth muscle cells，DNA electrophoresis showed that procyanidin in human coro- nary artery smooth muscle cells apoptosis in cell-specific DNA ladder bands．Procyanidin descendants coronary artery smooth muscle cells Caspase-9，Caspase-3 was significantly increased．Conclusion Proanthocyanidin may be through the mitochondri- al pathway inhibits human coronary artery smooth muscle cell growth and induced apoptosis． 【Key words】Procyanidin;Human coronary artery smooth muscle cells;Apoptosis 原花青素是植物中广泛存在的一大类多酚类聚合物的总 称，为双黄酮的衍生物，由不同数目的黄烷醇聚合而成。它在 自然界中广泛存在，主要存在于植物的果实、种子、花和外皮