TIP30在胃癌组织中的表达及其对胃癌血管生成的影响TIP30在胃癌组织中的表达及其对胃癌血管生成的影响.doc

TIP30在胃癌组织中的表达及其对胃癌血管生成的影响 【摘要】? 目的: 研究Tat作用蛋白30(Tat-interactive protein 30, TIP30)基因在胃癌组织中的表达及其对胃癌血管生成的影响。方法：运用免疫组织化学方法检测52例胃癌组织及47例癌旁正常组织中TIP30蛋白的表达水平，同时检测CD34标记的微血管密度(microvessel density，MVD)。结果: TIP30在胃癌和癌旁正常组织中的阳性率分别为53.8%和85.1%，两者相比较差异有统计学意义(χ2=11.22，P﹤0.01)，TIP30表达水平与患者年龄、性别、病理分化程度及肿瘤大小无关，与有无淋巴结转移及TNM分期有关。胃癌组织和癌旁正常组织中MVD值分别为27.37±2.68和21.87±4.11，两者比较差异有统计学意义(t=7.95，P0.01)，胃癌组织中TIP30表达阳性者较阴性者MVD值显著降低(t=-3.18，P=0.003)。结论: TIP30在胃癌组织中表达降低，其可能通过抑制胃癌血管生成影响胃癌的发生发展。 【关键词】? 胃肿瘤·新生血管化, 病理性·Tat作用蛋白30基因 　　【ABSTRACT】 Objective: To investigate the expression of TIP30 in gastric cancer tissues and analyze its impact with angiogenesis. Methods: The expression of TIP30 and CD34-labeled microvessel density(MVD) were measured by immunohistochemical staining in 52 cases of gastric carcinomas and 47cases of Para-cancer tissues. Results: the positive rate of TIP30 was 53.8% and 85.1% in cancerous tissues and para-cancer tissues, there were significant differences between them (χ2= 11.22, P0.01). The expression of TIP30 in gastric cancer was observed to correlate with lymph node metastasis and TNM stage, but not related with age, sex, cell differentiation and size of carcinoma. The MVD were 27.37±2.68 and 21.87±4.11 in cancerous tissues and para-cancer tissues.There were obvious differences( t= 7.95,P0.01 ).The MVD in TIP30-positive tissues was much lower than in negative tissues(t=-3.18，P=0.003 ).Conclusions: the expression level of TIP30 decreases obviously in gastric caicinoma. The effect of TIP30 gene for tumor genesis and growth may be partially completed through depression of vessel formation. 　　【KEY WORDS】 Stomach neoplasms·Neovascularization,pathologic·Tat-interactive protein 30 　　Tat作用蛋白30(Tat-interactive protein 30，TIP30/CC3)基因是国外学者Shtivelman等[1]新发现的一种与肿瘤转移抑制有关的基因。已有研究证明，TIP30蛋白本质是一种转录共分子，具有丝氨酸/苏氨酸激酶活性,可以通过调节RNA聚合酶Ⅱ的活化调节转录, 影响细胞的增殖和分化[2-3]。TIP30蛋白在肺癌、肠癌、乳腺癌及涎腺样囊性癌等肿瘤组织中的表达均较正常组织降低，提示TIP30蛋白与这些肿瘤的发生有关。本研究通过免疫组织化学方法观察TIP30蛋白在胃癌组织中的表达及其与微血管密度(microvessel density，MVD)的关系，探讨