盐胁迫下二倍体马铃薯叶绿素含量和抗氧化酶活性的变化..doc

盐胁迫下二倍体马铃薯叶绿素含量和抗氧化酶活性的变化摘要 在离体条件下研究盐胁迫对二倍体马铃薯富利亚（Solanum phurejaPHU）和窄刀薯（Sstenotomum，STN）杂种（PHU-STN）无性系相关生理指标的影响，以期探讨盐胁迫下二倍体马铃薯耐盐性的生理。在45份PHU-STN中，根据其在盐胁迫下六项生长参数的表现选出5份耐盐无性系、5份中耐盐无性系、5份感盐无性系，建立耐盐程度不同的三个组，以四倍体品种Bintje为耐盐对照，Mainechip为感盐对照，用0（CK），10，20，30 mmol·L-1的NaCl进行胁迫，测定叶绿素含量、和活性的变化。结果表明，随着盐浓度的升高，叶绿素含量呈的趋势，呈的趋势二倍体组间差异极显著；耐盐组的SOD活性呈升高的趋势，中耐盐组感盐组呈先降低后升高的趋势二倍体组间差异极显著POD活性呈先降低后升高的趋势二倍体组间差异极显著。因此，叶绿素含量、SOD和POD活性。 Change of Chlorophyll Content and Antioxidant Enzyme Activity of Diploid Potato under Salt Stress Abstract Physiological traits were studied of diploid potato Solanum phureja (PHU) and S. stenotomum (STN) hybrid (PHU-STN) clones in vitro under salt stress in order to understand the physiological performance in salt tolerance of diploid potatoes. Three groups of five salt tolerant, five medium salt tolerant and five salt susceptible clones were selected based on their performances of six growth parameters in salt stress in 45 clones, and tetraploid variety Bintje (salt tolerant) and Mainechip (salt susceptible) were also included in this experiment. They were stressed in vitro at 0 (CK), 10, 20 and 30 mmol·L-1 salt concentrations, and chlorophyll content, Superoxide Dismutase (SOD) and Peroxidase (POD) activities were measured. With increase in salt concentration, chlorophyll content in slat tolerant group was increased, and then decreased, while chlorophyll content in medium salt tolerant group and salt susceptible group showed decreasing trend. Chlorophyll contents in all groups were found to be of highly significant difference. SOD activity in salt tolerant group showed increasing trend with increase in slat concentration, while for medium salt tolerant group and susceptible group it was decreased, and then increased. Difference among groups were high significant. For POD activity, it was decreased and then increased in salt tolerant group and medium salt tolerant group with increase in salt concentrations, but showed decreasing trend in salt suscep