武汉理工大学试题.doc

武汉理工大学考试试题纸（? A卷） 课程名称 细胞工程 专业班级理学院生物系04级 题号 一 二 三 四 五 六 七 八 九 十 总分 题分 ?10 ?10 ?10 ?20 ?20 30 ? ? ? ? ?100 ??????????????? 备注:?? 学生不得在试题纸上答题(含填空题、选择题等客观题) 一、单项选择题（请选出唯一正确的答案，每题2分，共10分） 　　1、From what time modern biotechnology developed? 　　A. 1960s; B. 1970s; C. 1980s; D. 1990s. 　　2、Which of the following is not advantage of using plant cell culture to produce secondary metabolites? 　　A．Unlimited to environmental factors；B．Better Quality and quantity of secondary metabolites； 　　C．Not affected by human and social factors；D．Cheap. 　　3、Which of the following is not used in transferring foreign gene into animals? 　　A．Embryonic stem cell-mediated gene transfer；B．DNA microinjection；C．Retrovirus-mediated gene transfer；D．Sperm mediated gene transfer；E．Gene gun；F．Cell nuclear transfer. 　　4、以下哪一项不是在细胞工程实验室工作时必须遵守的制度 　　A．准入制度；B．出入制度；C．安全制度；D．保卫制度；E．卫生消毒制度 　　5、Which of the following is not morphological propertie of stem cells? 　　A． round shape; B．small volume；C．low ratio of nuclear to cytoplasm；D．high telomerase activity 二、多项选择题（请选出所有正确的答案，每题2分，共10分） 　　1、Plant cell engineering could be used in: 　　A．Plant breeding; B．Plant virus elimination and micropropagation; C．Producing plant secondary metabolites; D．genetic, physiological, biochemical research in plants; E．Producing antibodies. 　　2、Cryopreservation aims for: 　　A. preserving plant material over a longer period; B. minimize growth and development in vitro；C．maintain viability and genetic stability；D．maintain full developmental and functional potential；E. saving of labour input 　　3、A broad meaning of plant tissue culture is an aseptic culture of: 　　A．embryo；B．organ；C．tissue；D．cell；E．protoplast； 　　4、Which of the following could be used to index plant virus? 　　A． PCR；B．Inoculation of virus onto host species；C．Serological tests；D．Electron microscopy；E．Nucleic acid hybridization; F．ELISA 　　5、What are major problems of somatic cell cloning technique? 　　A．Unstable and low efficiency；B．High mortality rate of offspring；C．do