生物化学结课论文生物化学结课论文.doc

生 物 化 学 结 课 论 文 学 院：物理化学学院 姓 名：刘双双  学 号：311113030202 专业班级：应用化学11-02 授课老师：斯琴格日乐 成 绩： 目录 摘 要............................................................3 关键词............................................................3 前 言............................................................4 第一章 概论......................................................4 第二章 总糖及还原糖提取分离及纯化方法的研究..............4 2.1 总糖及还原糖的提取.........................................4 2.2 总糖及还原糖的分离.........................................5 2,3 总糖及还原糖的纯化.........................................5 第三章 实验部分.................................................6 3.1 实验仪器及药品.............................................6 3.2 实验步骤...................................................7 3.3 实验数据处理...............................................8 第四章 结论与展望..............................................9 致 谢..........................................................9 参考文献.........................................................10 淀粉中提取总糖及还原糖的研究 【摘 要】：本文利用3,5－二硝基水杨酸法测定淀粉中的总糖和还原糖。掌握还原糖和总糖的测定原理，用比色法测定还原糖的方法在和丙三醇存在下，3,5－二硝基水杨酸（DNS）与还原糖共热后被还原生成氨基化合物。在过量的NaOH碱性溶液中此化合物呈桔红色，在540nm波长处有最大吸收，在一定的浓度范围内，还原糖的量与光吸收值呈线性关系，利用比色法可测定样品中糖量。3,5－二硝基水杨酸 【 】 : in this paper, using the method of 3, 5-2 nitro salicylic acid determination of total sugar and reducing sugar in starch content. To master the principle of the determination of reducing sugar and total sugar, and the method of reducing sugar was determined by colorimetric method. Its principle is: in the presence of NaOH and glycerol, 3, 5-2 nitro salicylic acid (DNS) after thermal reduction with reducing sugars to generate amino compounds. NaOH in excess of the compounds in alkaline solution are orange, had the biggest absorption, in the 540nm wavelength in a certain concentration range, the amount of reducing sugar