[药用真菌发酵及其产物对AA肉鸡免疫功能及生长的影响.doc

药用真菌发酵及其产物对AA肉鸡免疫功能及生长的影响 摘要:以一药用真菌进行固体发酵,比较不同培养基质、起始含水量、起始pH值对其生长的影响;再以该真菌发酵物(菌质)作为饲料添加剂试验,研究其对AA肉鸡生长性能和免疫功能的影响。结果显示:以大米、小米和复合基质进行固体发酵,该真菌生长较好(生物量为3518% ),且添加一定量的中药不影响真菌的生长,起始含水量为50%,起始pH值为415;动物试验表明,含中药的发酵产物能明显增强AA肉鸡对新城疫,禽流感H9、H5的免疫力(P0105,P0101),体增重显著高于对照组(P0105,P0101)。 关键词:药用真菌;免疫力;肉鸡;体增重 Studies on medicinal fungi fermentation and effects of its products on the mi munity function and growth performance ofAA broiler ZHANG L-i yang, ZHOUYe-fe,i ZHANG Dun-lin (Life Science DepartmentofNanjingXiaozhuang Institute, Nanjing 210017, China) Abstract:The solid fermentation with pharmaceutical fungiwas studied. The effects of differentmedium, initialwater content and initial pH on growth ofpharmaceutical fungiwere compared. Themedicinal fungal substance by solid fermentationwith some conditionswas acted as feed additive, then the effectofmedicinal fungalon immune function and growth performance ofAA broilerwas studied. The results indicated that the growth of fungiwith solid fermentation under the rice, milletand compositemedium was betterwith its biomass of3518%. SomeCh-i nesemedicine in the fermentationmedium didnctaffect fungigrowthwhen the initialwater contentand initialpHwere 50% and 415. Fermen- tation product of fung,i including Chinese medicine, could enhance AA broilercs immunity against Newcastle disease (ND ), H9 收稿日期: 2004-10-24 基金项目:江苏省教育厅科技项目(2004nxy01)。 作者简介:张李阳(1961-),女,副教授,硕士。 因的大肠杆菌繁殖时,带有淀粉酶基因的质粒随着分裂进入到 新的细胞体内。故在大肠杆菌大量繁殖时,淀粉酶活力也随着 提高。③大肠杆菌分泌淀粉酶的机制可能优于枯草杆菌。 本试验中,选择了2种培养液来增殖细菌。LB是一种常 用的细菌培养液,似乎对原始枯草杆菌淀粉酶分泌有促进作 用。矿物元素培养液对转基因的大肠杆菌分泌淀粉酶能力有促 进作用,这可能是淀粉诱导淀粉酶产生的原因。因而在实际生 产中,要考虑对不同的菌种选择最适宜的培养液,以便于最大 限度地提高淀粉酶的产生。 参考文献: [1] 张树政.酶制剂工业[M].北京:科技出版社, 1984, 466- 467. [2] Lin L L, HsuW H. Lactose-induced expression ofBacillus sp. TS- 23 amylase gene inEscherichia coliregulated by aT7 promoter [J]. Letters in AppliedMicrobiology, 1997, 365(1): 235-239. [3] AusubelFM, BrentR, Kingston R E, et a.l ShortProtocols inMo- lecularBiology. Third edition [M]. New York: John W