aromatic hydrocarbon Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-nu.doc

aromatic hydrocarbon Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-nu 导读：就爱阅读网友为您分享以下“Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-nu”资讯，希望对您有所帮助，感谢您对92的支持! Wrenn BA, Venosa AD. Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-number procedure[J]. Canadian journal of microbiology. 1996, 42(3): 252-258. Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most probable number procedure Brian A. Wrenn and Albert D. Venosa Abstract: A most-probable-number (MPN) procedure was developed to separately enumerate aliphatic and aromatic hydrocarbon degrading bacteria, because most of the currently available methods are unable to distinguish between these two groups. Separate 96-well microtiter plates are used to estimate the sizes of these two populations. The alkane-degrader MPN method uses hexadecane as the selective growth substrate and positive wells are detected by reduction of iodonitrotetrazolium (碘硝基四氮唑)violet, which is added after incubation for 2 weeks at 20°C. Polycyclic aromatic hydrocarbon degraders are grown on a mixture of phenanthrene, anthracene, fluorene, and dibenzothiophene in a second plate. Positive wells turn yellow to greenishbrown from accumulation of the partial oxidation products of the aromatic substrates and they can be scored alter a 3-week incubation period. These MPN procedures are accurate and selective. For pure cultures, heterotrophic plate counts on a nonselective medium and the appropriate MPN procedure provide similar estimates of the population density. Bacteria that cannot grow on the selective substrates do not produce false positive responses even when the inoculum density is very high. Thus, this method, which is simple enough for use in the field, provides reliable estimates for the density and composition of hydrocarbon-degrading mic