variety 2015-NS-功能分析-Generation of a variety of stable_图文.doc

variety 2015-NS-功能分析-Generation of a variety of stable_图文 导读：就爱阅读网友为您分享以下“2015-NS-功能分析-Generation of a variety of stable_图文”的资讯，希望对您有所帮助，感谢您对92的支持! /scientificreports OPEN Received: 21 January 2015 accepted: 21 May 2015 Published: 12 June 2015Generation of a variety of stable Influenza A reporter viruses by genetic engineering of the NS gene segment Peter Reuther1,2,*, Kristina G?pfert1,*, Alexandra H. Dudek1,2,3, Monika Heiner4, Susanne Herold4 amp; Martin Schwemmle1 Influenza A viruses (IAV) pose a constant threat to the human population and therefore a better understanding of their fundamental biology and identification of novel therapeutics is of upmost importance. Various reporter-encoding IAV were generated to achieve these goals, however, one recurring difficulty was the genetic instability especially of larger reporter genes. We employed the viral NS segment coding for the non-structural protein 1 (NS1) and nuclear export protein (NEP) for stable expression of diverse reporter proteins. This was achieved by converting the NS segment into a single open reading frame (ORF) coding for NS1, the respective reporter and NEP. To allow expression of individual proteins, the reporter genes were flanked by two porcine Teschovirus-1 2A peptide (PTV-1 2A)-coding sequences. The resulting viruses encoding luciferases, fluorescent proteins or a Cre recombinase are characterized by a high genetic stability in vitro and in mice and can be readily employed for antiviral compound screenings, visualization of infected cells or cell