树舌灵芝多糖的提取及其抗氧化作用..doc

江淮学院本科毕业论文（设计） 题　　目　　　　树舌灵芝多糖的提取及其抗氧化作用　 　　　　　 　　　 　　　 　　 　 　　　　　　　　　 　　　 学生姓名：　　　 司楠楠 学号 系 别：　　 理工部　　　 专业：　 生物科学　　 入学时间：　 　 2012　　　　　年　　 09　　　月 导师姓名：　 吴庆喜　　 　　 职称/学位：讲师/博士 　 毕业论文（设计）提交时间：二○一六　　 　　年 四 月 安徽大学江淮学院教学科研部　制 树舌灵芝的提取及其抗氧化作用 摘 要 关键词：树舌灵芝多糖；单因素分析；正交试验；抗氧化作用  Extraction and antioxidation stud of polysaccharides from Ganoderma lucidum Abstract Objective: To study the extraction conditions and antioxidation of Ganoderma lucidum polysaccharides from Ganoderma lucidum. Methods: in this experiment, the water extraction and alcohol precipitation method for the extraction of Ganoderma lucidum polysaccharides, and analysis of single factor experiments and orthogonal test of extraction process of solid to liquid ratio, extraction time, extraction temperature, to polysaccharide yield as the index to get the best extraction conditions. In this paper, we selected two indicators, namely, the scavenging effect of DPPH and the scavenging effect of -OH on the antioxidant activities of Ganoderma lucidum polysaccharides. Results: orthogonal test results showed that the best extraction conditions: extraction temperature was 90 degrees C, extraction time was 2h, the ratio of material to liquid ratio of 1:15 was 69.7%. Conclusion: the effects of the extraction factors on the extraction rate were as follows: extraction temperature feed liquid ratio. The Ganoderma lucidum polysaccharides had strong scavenging effect on DPPH and -OH, and had a strong dependence on the concentration, which indicated that the Ganoderma lucidum polysaccharides had strong antioxidant activity. Key words: Ganoderma lucidum polysaccharides; Single factor analysis; Orthogonal test; Antioxidation 目录 第一章引言 第二章 材料、试剂、仪器 第三章 方法 3.1 原材料的处理 将部分野生树舌灵芝用打碎机将其打碎呈粉末状，用保鲜袋分装好存放于冰箱中。 3.2 树舌灵芝多糖的提取工艺路线 取灵芝粉末→热水法浸提多糖→过滤去除滤渣→收集上清液→上清液浓缩→浓缩液中加乙醇沉淀→离心→收集沉淀