微生物大实验实验报告(自动保存的)..docx

中央民族大学生命与环境科学学院微生物综合性设计实验报告化学抑杀菌剂的效果评价Evaluation of bactericide effect of chemical inhibition姓名：杨芳学号： 1245015 年级： 12级专业：生物科学类一班小组成员：胡雪辰、付国松、王新凯指导教师：李崴2013年11月30日卡那霉素、氨苄青霉素和四环素对细菌抑杀效果的评价杨芳（中央民族大学生命与环境科学学院 北京 100081）摘要：目的了解抗生素对微生物生长的影响，学习抗菌谱试验的基本方法；自主设计方案，掌握评价化学抑杀剂作用效果方法；掌握评价化学抑杀剂作用效果方法。方法本实验主要采用倾注法测菌悬液浓度、滤纸片法测定抗生素的最小抑制浓度（MIC）及pH值对抗生素作用效果的影响和t检验法对实验结果进行统计学分析。结果本次试验所用大肠杆菌和枯草芽孢杆菌菌悬液浓度分别为1.3107CFU/mL、7.1107CFU/mL；对大肠杆菌卡那霉素、氨苄青霉素和四环素的MIC分别为0.006mg/mL、0.098 mg/mL和0.781mg/mL，对枯草芽孢杆菌卡那霉素、氨苄青霉素和四环素的MIC分别为0.024 mg/mL、0.391 mg/mL和0.195mg/mL；卡那霉素对大肠杆菌为杀灭作用，对枯草芽杆菌为抑制作用、四环素对大肠杆菌为抑制作用，对枯草芽孢杆菌为杀灭作用、青霉素对枯草芽孢杆菌为抑制作用，因大肠杆菌出现抗性，所以青霉素对大肠杆菌的作用不明显。结论对同一药剂，低浓度的药剂对细菌抑杀效果不明显而随着浓度的增大其抑杀效果越来越明显；同一浓度的同一药剂对不同细菌有不同的抑杀效果；不同药剂对同一细菌的抑杀效果是不同的。关键字：最小抑制浓度；抗生素；大肠杆菌；枯草芽孢杆菌Kanamycin, ampicillin and tetracycline evaluation of the inhibition of bacterial killing effectsYang fang(College of life and environmental science, Minzu University of china, Beijing,100081 )Abstract：Objectiveunderstand the impact of antibiotics on microbial growth, learn basic methods of spectrum antimicrobial testing; design independently, master evaluate effects of chemical agents to kill suppression methods; grasp evaluate effects of chemical agents to kill suppression methods.MethodsThis experiment measured use pour bacterial suspension concentration, filter paper assay antibiotic minimum inhibitory concentration (MIC) and the effect of pH on the effects of antibiotics and t-test of the experimental results were analyzed statistically.ResultsThe tests used Escherichia coli and Bacillus subtilis bacteria suspension concentration was 1.3107CFU/mL, 7.1107 CFU / mL.Escherichia coli kanamycin, ampicillin and tetracycline MIC was 0.006 mg / mL, 0.098 mg / mL and 0.781mg/mL.Bacillus subtilis, kanamycin, ampicillin and tetracycline MIC was 0.024 mg / mL, 0.391 mg / mL and 0.195mg/mL; kanamycin killing effect on E. coli, Bacillus subtilis for the inhibition of E. coli tetracycline inhibition of Bacillus subtilis to kill, penicillin