系统分析臭氧处理过的污泥的微生物生化性能和生物群落的变化..doc

Abstract: Two lab-scale bioreactors (reactors 1 and 2) were employed to examine the changes in biological performance and the microbial community of an activated sludge process fed with ozonated sludge for sludge reduction. During the 122 d operation, the microbial activities and community in the two reactors were evaluated. The results indicated that, when compared with the conventional reactor (reactor 1), the reactor that was fed with the ozonated sludge (reactor 2) showed good removal of COD, TN and cell debris, without formation of any excess sludge. In addition, the protease activity and intracellular ATP concentration of reactor 2 were increased when compared to reactor 1, indicating that reactor 2 had a better ability to digest proteins and cell debris. DGGE analysis revealed that the bacterial communities in the two reactors were different, and that the dissimilarity of the bacterial population was nearly 40%. Reactor 2 also contained more protozoa and metazoa, which could graze on the ozone-treated sludge debris directly. 摘要： 利用两个实验室规模的生物反应器（反应器1和2)加以检验活性污泥工艺运用污泥臭氧氧化分解法的与否，生物学性能和生物群落的变化及活性污泥法处理污泥减少的效果。在122天的操作过程中,对两个反应器在微生物活动和群落进行评估。结果表明，与常规反应器（1)相比,输送臭氧氧化污泥的反应器（2）表现出了更好的COD、TN和细胞碎片去除效果,并且没有形成任何多余的污泥。此外, 反应器2较反应器1中蛋白酶活性和细胞内ATP浓度增加更多，且反应器2具有更好的消化蛋白质和细胞碎片的能力。DGGE分析显示两个反应器不同细菌群落数接近40%。反应器2又含有较多的原生动物、后生动物,能直接吃掉经过臭氧处理过的污泥的碎片。 Introduction: The use of activated sludge as a biological wastewater treatment process has been employed to treat a wide variety of wastewater.However, its primary by-product, excess sludge, has become a large problem. Indeed, treatment and disposal of excess sludge can account for up to 60% of the total operational costs of a wastewater treatment plant (Spellman, 1997). For this reason, it is important to develop methods of reducing excess sludge produced during wastewater treatment in an economic, environmentally safe and practical manner. One promising technique is the sludge ozonation process (Yasui et al., 1996; Wei et al., 2003; Yan