小鼠大脑中动脉栓塞模型的改良及评价【摘要】目的对小鼠大脑中动脉.docVIP

小鼠大脑中动脉栓塞模型的改良及评价 【摘要】目的：对小鼠大脑中动脉栓塞（MCAO）模型进行改良并进行评价。方法：C57BL/6J小鼠50只，随机分为假手术组10只，MCAO组40只；改良线栓法制作小鼠MCAO模型；术后24 h行神经功能评分，TTC染色测脑梗死体积；术后30 d，水迷宫测试小鼠远期学习和记忆能力；术后90 d，头部MRI观察梗死病灶及脑组织变化。结果：造模成功率为67.5％；MCAO组小鼠神经功能评分为（2.36±0.21）分，相对梗死体积为（25.36±3.65）％；MCAO组小鼠逃避潜伏期明显长于假手术组，穿越原平台位置次数明显少于假手术组（P0.05）；小鼠头部MRI T2加权像可见缺血侧皮质和/或皮质下梗死病灶。结论：线栓法是简单、可靠的制作小鼠MCAO模型的方法。 【关键词】小鼠；大脑中动脉栓塞；动物模型 Establish and optimize middle cerebral artery occlusion model in mice Tang Ying-xin▲, LIU Min-zhen, DING Feng-fei, ZHANG Qiang, WANG Wei, PAN Deng-ji. ▲Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 【Abstract】Objective: To establish and optimize middle cerebral artery occlusion model in mice. Methods: Fifty C57BL/6J mice were randomly divided into sham group (n=10) and MCAO group (n=40). The mice in MCAO group were used to establish the MCAO model by intraluminal occlusion using monofilament. Twenty-four hours after operation, the nervous function was evaluated and the infarct volume was calculated by TTC staining. Thirty days after operation, Morris water maze test was carried out to investigate spatial learning and memory of mice. Ninety days after operation, head MRI scan was conducted. Results: In MCAO group, the successful rate of execution was 67.5%. The neurological deficit score was (2.36±0.21) and the relative infarct volume was (25.36±3.65)% in MCAO group. Mice in sham group performaced better in water maze task than those in MCAO group. T2-weighted MRI imagines showed infarction clearly. Conclusion: Intraluminal occlusion of MCA using monofilament is a kind of simple and reliable method to establish mouse MCAO model. 【key words】mice; middle cerebral artery occlusion; animal model 大脑中动脉栓塞（middle cerebral artery occlusion, MCAO）模型是目前使用最为广泛的、研究局灶性脑缺血再灌注损伤的理想模型。线栓法[1]是制作MCAO模型常用方法，大鼠是最常用的模型动物。随着基因技术的发展，越来越多的研究选择转基因或基因敲除小鼠为实验动物，但关于构建小鼠MCAO模型的研究不多[2,3]。本研究对小鼠MCAO模型进行一定的改良并进行相关评价。 1 材料与方法 1.1 材料 1.1.1 实验