高效液相色谱法测定水煎法提取中成药中的甘草酸含量..doc

高效液相色谱法测定水煎法提取中成药中的甘草酸含量. nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：杜学勤，刘焕蓉，郭志强，邓并 【摘要】nbsp; 目的建立HPLC法测定以水煎法提取19种中成药中甘草酸（GA）的含量。方法采用日本岛津PC8色谱柱；流动相为甲醇-水-醋酸（57.0∶42.6∶0.4）；检测波长为254 nm；流速2.0 ml·min-1。结果甘草酸在200～2000 μg·ml-1，r=0.999 7的浓度范围内线性关系良好，平均回收率为96.55%，RSD=1.1%；同时研究了22种单味药对甘草中GA含量测定的影响。结论 通过与冷浸法比较，水煎法和冷浸法提取中成药中GA含量差值较大，且水煎法的偏低。 【关键词】nbsp; 中成药；甘草酸；高效液相色谱法；水煎法 　　Determination of Glycyrrhizic Acid in the Water Extraction in Chinese Patent Medicine by HPLC 　　Abstract：ObjectiveAn HPLC method was established for the determination of glycyrrhizic acid (GA) by extracting method of decoction in 19 kinds Chinese patent medicine prepared in laboratory.Methods PC8 column was used with methanol-water-cuetu acid (57.0∶42.6∶0.4) as mobile phase. The flow rate was 2.0ml·min-1 and detection wavelength was 254nm.ResultsThe linear relationship was good when the content of glycyrrhizic acid (GA) was in the range of 200～2 000 μg·ml-1（r=0.999 7）.The average recovery and RSD were 96.55% and 1.1% respectively. The paper also studied the interference determination of GA by selecting interrelation 22 kinds of single medicinal material.ConclusionBy comparison the decoction with the infusion ，the results shows that the content of GA by extraction decoction was lower than that by infusion. 　　Key words： Chinesenbsp; patent medicine ;nbsp;nbsp; Glycyrrhizic acid;nbsp;nbsp; HPLC;nbsp; Decoction nbsp;nbsp;nbsp; 甘草为豆科植物甘草Glycyrrhizanbsp; uralensis Fisch.，胀果甘草Glycyrrhizanbsp; inflata Bat.或光果甘草Glycyrrhiza glabra L.的干燥根及根茎，具有补脾益气、清热解毒、祛痰止咳、缓急止痛、调和诸药之功［1］，被广泛用于中药制剂处方中。甘草中主要活性成分为甘草酸［2］(glycyrrhizic acid，GA)，因此,我们选定甘草酸作为含甘草中成药的质量控制指标。甘草酸的含量测定方法报道很多，但大多数方法操作复杂，定量不够精确。近年来, 高效液相色谱法测定GA也已有报道［3～6］，但对水煎法提取中成药中GA含量测定方法尚未见报道。本文在冷浸法研究的基础上采用高效液相色谱法测定水煎生药中的GA和不同剂型中药复方制剂中的GA含量，为评价含GA药材及其制剂的质量提供了一个准确、灵敏、快速的测定方法。 　　1nbsp; 仪器与试药 　　1.1nbsp; 仪器nbsp; 　　日本岛津LC3A型高效液相色谱仪；日本岛津SPD-1型紫外吸收检测器；日本岛津CRIB型色谱仪数据处理机。 　　1.2nbsp; 试剂无水甲醇、冰醋酸（均为分析纯，北京化工厂生产）；甘草酸铵盐对照品：（旋光度［a］20D60.5熔点209℃）纯度88.00%（