《a淀粉酶的生产工艺.docVIP

宜宾职业技术学院 论文 α-淀粉酶的生产工艺 系 部 生物与化工工程系 专 业 名 称 酿 酒 技 术 班 级 酿 酒11103 姓 名 王梦韵 李艳梅 严红俊 学 号 201117315 201117404 201117346 指 导 教 师 兰 小 艳 二〇一二年十月二十九日 α-淀粉酶的生产工艺 摘要 α-淀粉酶广泛分布于动物、植物和微生物中，能水解淀粉产生糊精、麦芽糖、低聚糖和葡萄糖等，是工业生产中应用最为广泛的酶制剂之一。目前，α-淀粉酶已广泛应用于变性淀粉及淀粉糖、焙烤工业、啤酒酿造、酒精工业、发 酵以及纺织等许多行业。本次设计的淀粉酶发酵，分别以玉米粉为碳源，以豆饼为氮源，以BF-7658枯草芽孢杆菌为生产菌种,同时做出了生产工艺流程图，详细的介绍了α-淀粉酶的生产工艺。 关键词：α-淀粉酶；生产工艺设计；深层发酵法 Alpha amylase production technology Abstract Alpha amylase widely distributed in animals, plants and microbes, can be hydrolyzed starch produce dextrin, maltose, oligosaccharide and glucose and so on, it is the industrial production in the most widely used one of enzyme preparation. At present, the alpha amylase has been widely used in modified starch and starch sugar, baking industry, brewing, alcohol industry, hair leaven and textile and many other industries. The design of amylase fermentation, respectively with corn flour for carbon source, nitrogen source for bean cake, with BF - 7658 bacillus subtilis strains for the production, at the same time, make the production process flow diagram, detailed introduces the alpha amylase production technology. Keywords: Alpha amylase, Production process design; Deep fermentation 目录 前言................................................................................................................................1 1 α-淀粉酶的生产方法..................................................................................................1 1.1 生产方法的选择...................................................................................................1 1.2 α-淀粉酶培养基的制作....................................................................3 1.2.1耐高温α-淀粉酶的生产方法............................................................................4 1.2.2耐高温α－α－淀粉酶α－淀粉酶枯草芽孢杆菌α－淀粉酶α-淀粉酶分离纯化............................................................