酸棗和灵武红枣中总生物碱含量的紫外分光光度法测定.docVIP

酸枣和灵武长枣中总生物碱含量的紫外分光光度法测定 摘 要 [目的]对酸枣与灵武长枣的枣肉和枣核的总生物碱含量测定方法进行比较研究，以期对酸枣和灵武长枣的开发利用提供技术参考。[方法]利用酸提碱沉法对酸枣与灵武长枣枣肉和枣核的总生物碱进行粗提取，并用无水乙醇萃取可得生物碱的粗提物。采用紫外分光光度法，以莲心碱为对照品，测定枣肉与枣核中的总生物碱含量。[结果]莲心碱对照品在浓度（0.006-0.03mg/ml ）范围内线性关系良好，标准曲线为y=11.029x-0.0038。R2=0.9918。RSD为2.51%，精密度与重复性良好。总生物碱含量灵武长枣枣肉为0.018%，灵武长枣枣肉为0.020%，酸枣肉为0.0183%，酸枣核为0.531%。[结论] 酸枣核中的总生物碱含量远高于酸枣肉和灵武长枣枣核与枣肉。生物碱的含量是枣核中的高于枣肉。酸枣和灵武长枣总生物碱含量由高而低依次为酸枣核灵武长枣枣核酸枣枣肉灵武长枣枣肉。采用紫外分光光度法能够简便准确的测定出酸枣与灵武长枣总生物碱的含量。 关键词：酸枣，灵武长枣，总生物碱，紫外分光光度法 STUDY ON THE CONTENT OF TOTAL ALKALOIDS IN SEMEN ZIZYPHI SPONOSAE AND ZIZIPHUS ZIZYPHUS BY UITRAVIOLET VISIBLE SPETROPHOTOMETRY ABSTRACT [Objective] A comparative study of the determination method of total alkaloid content of jujube and purpose of Lingwu long jujube jujube flesh and seeds, in order to provide technical reference for the development and utilization of wild jujube and jujube. The total alkaloids [method] using acid alkali extraction precipitation on Lingwu long jujube jujube and jujube flesh and seeds of crude extraction, and ethanol extraction can be alkaloids extracts. UV spectrophotometry was used, the Liensinine as standard, determine the content of total alkaloids in jujube flesh and seeds. [results] Liensinine reference substance in concentration (0.006-0.03mg/ml) linear relationship is good, the standard curve was y=11.029x-0.0038. R2=0.9918. RSD was 2.51%, precision and good repeatability. The total alkaloid content of Lingwu long jujube jujube meat is 0.018%, Lingwu long jujube jujube meat is 0.020%, jujube meat is 0.0183%, jujube kernel 0.531%. [Conclusion] the total alkaloid content of jujube nucleus than jujube jujube meat and Lingwu long jujube seed and jujube meat in the amount of. The content of alkaloid is way in is larger than the amount of jujube meat. The content of total alkaloids in order of Lingwu long jujube jujube jujube nucleus jujube jujube meat Lingwu long jujube jujube meat. Method for the determination of the more ac