重金屬离子的固相萃取和分离技术—毕业论文.doc

重金属离子的固相萃取和分离技术 摘要：模板0℃烘箱反应24h制得整体柱。基于上述的印迹整体柱，对一系列标准浓度的溶液进行测试。整体整体柱：；；印迹；整体柱Abstract: Epoxy resin based ion imprinted polymer was prepared by heating a kind of uniformly mixed mixture in an electric oven, whose temperature is kept at 60℃.The mixture that contain epoxy resin takes Ni2+,Co2+,Cd2+ and Pb2+ as template ions, PEG-1540 as pore-creating material and diethylene triamine as curing agent. The present study takes the absorption ability of ion imprinted polymer and its appropriate scale problem as the goal in research is carrying out adsorption experiments in which a series of heavy metal ion standard solutions are adsorbed by ion imprinted polymer. Considered the influence of the monolithic materials synthesis conditions and adsorption conditions，the adsorption properties of the monolithic material will be tested in different conditions in order to optimize the parameters of the ion imprinted polymer. Keywords：solid-phase extraction; metal ions; ion imprinting; monolithic column 目 录 1. 引言……………………………………………………………………………….....1 1.1. 背景与研究意义…………………………………………………………….…1 1.2 固相萃取的发展……………………………………………………….…….....2 1.2.1 固相萃取…………………………………………………………….…..2 1.2.2 分子印迹技术……………………………………………………….…..3 1.2.3 整体柱…………………………………………………….……………..3 2. 实验部分………………………………………………………………….………….5 2.1 实验试剂与仪器………………………………………………………………5 2.1.1 试剂………………………………………..…………………..……….5 2.1.2 实验仪器……………………………………………………….....……5 2.2 离子印迹整体柱的合成………………………………….……………...……5 2.2.1 称取合成各离子印迹柱所需结晶水化合物的质量………….………5 2.2.2 制备重金属离子印迹整体柱……………………………….…………6 2.3 整体柱处理………………………...………………………………….………6 2.3.1 PEG-1540的去除……………………………………………….…...…6 2.3.2 整体柱中印迹离子的去除…...……………………………….……….7 2.4 重金属离子标准溶液的配制…..……………………………………..………7 2.4.1各离子标准溶液的配制………………………………………………..7 2.4.2 混合标准溶液的配制………………………………………………….7 2.4.3金属离子标注曲线方程测定…………………………………………..8 2.5 整体柱各项性能的测试………………………………………………………8 2.5.1整体柱吸附量与选择吸附性测………………………………………..8 2.5.2 吸附性能空白对比实验……………………………………………….9 2.5.3 最大吸附量实验……………………………………………………….9 2.5.4 单独进行铅离子印迹整体柱的吸附性实验……………………….…9 2.5.5 铅离子印迹整体柱空白实验………