安溪茶树根际微生物多样性的PCR-DGGE法研究.docVIP

茶园根际土壤细菌群落结构多样性 赵艳1 胡桂萍1 陈李林1 尤民生1 1福建农林大学应用生态研究所，福建福州，350002 摘 要采用PCR-DGGE分子指纹图谱技术分析安溪铁观音种植区不同海拔茶园根际土壤细菌群落结构。结果表明，安溪铁观音种植区茶园根际土壤的16S rDNA的扩增结果约为1500 bp, V3区为230bp。茶园根际土壤中大约有14种优势菌群，对DGGE图谱中的14条主带进行回收，扩增和测序，显示11个条带代表的细菌是不可培养的, 3个条带代表的细菌是可培养的，分别属于根瘤菌属（Rhizobium）、中华根瘤菌属（Sinorhizobium）和苍白杆菌属（Ochrobactrum）。DGGE聚类分析得到，同一海拔下茶园根际土壤群落结构相似不同海拔茶园根际土壤群落结构差异。Shannon-Wiener多样性指数分析表明，400 m海拔处茶园土壤细菌多样性最高。蒙特卡罗检验分析得到环境因子协同作用对安溪铁观音茶园土壤群落结构贡献为59.6%，其中环境因子（P）贡献为10.6%冗余分析也显示安溪茶园根际土壤群落结构与海拔相关。茶园根际土壤，为铁观音的生长的健康管理提供微观理论基础。 关键词：茶园；根际；群落结构；变性梯度凝胶电泳 Diversity of Community in Tea Rhizosphere Soil ZHAO Yan1 HU Gui-Ping1 SONG Feng-Qin1 CHEN Li-Lin1 YOU Min-Sheng1* 1 Institute of Applied Ecology, Fujian Agriculture and Forest University, Fujian Fuzhou, 35000) Abstract:【Background】The diversity of bacteria community structure is closely related to the nutrient cycling of tea rhizosphere soil, and changes in its composition can be used as a biological symbol for healthy tea garden. 【Method】Bacterial communities of the rhizosphere soils were analyzed by DGGE-PCR, and the rhizosphere came from tea planting areas at different altitudes in Anxi County. 【Result】The results showed that the size of the 16S rDNA sequence amplified from tea rhizosphere soils was approximately 1500 bp, including 230 bp of the V3 region. The DGGE profiles of rhizosphere soils at different altitudes are significantly and about fourteen types of microorganisms are identified. The major bands of 16S rDNA genes fragments from profiles were further eluted, reamplified and sequenced, the sequences of the fragments were compared with the database in GeneBank, the results displayed that 11 fragments belonged to uncultured bacteria. Another 3 belonged to genius of Rhizobium, Sinorhizobium and Ochrobactrum, respectively. Clustering of DGGE revealed that microorganism community of tea rhizosphere soil are very similar at the same altitude, and The highest bacterial diversity was found at 400 meters based on calculating t