【2017年整理】中药白芨促进角质形成细胞的游走.docx.docxVIP

网址： HYPERLINK 第 PAGE 1 页，共 NUMPAGES 1 页 中药白芨促进角质形成细胞的游走 中药白芨促进角质形成细胞的游走 中华皮肤科杂志 1999年第3期第32卷 论著 作者：陈德利　施伟民　徐　倩　高青云　朴英兰　沈亮亮 单位：200065上海铁道大学附属甘泉医院皮肤性病科 关键词：单方(中药)白芨角蛋白细胞器官培养 【摘要】目的　探讨中药白芨对角质形成细胞游走的影响及其对创伤愈合的作用机制。方法　采用皮肤器官培养法(浮游法)培养SD小鼠皮片，比较观察了含不同浓度白芨的培养基对角质形成细胞游走的影响及培养时间与游走长度的关系。结果　发现白芨浓度为2×10－2mg/mL及2×10－3mg/mL时，角质形成细胞游走比对照组显著地快和长。含白芨组更早地显示游走迹象，较对照组早4h。培养48h内，白芨组及对照组中培养时间与游走长度皆呈直线正相关，白芨组的促游走作用显著地优于对照组。结论提示白芨有明显的促进角质形成细胞游走作用，这种促游走作用可能对治疗皮肤创伤早期愈合有重要影响。 Rhizoma Blelillae Promotes the Migration of Keratinocyte CHEN Deli, SHI Weiming,XU Qian, et al.Department of Dermatology, Ganquan Hospital, Shanghai Tiedao University, Shanghai 200065 【 Abstract】 Objective To investigate the effect of Rhizoma Bletillae(RB) on keratinocyte migration and its mechanism in the treatment of skin wound.Methods An organ culture technique of skin was applied to cultivate the skin explants of SD mice and a comparative analysis was processed to study the effect of RB on keratinocyte migration and the relationship between duration of culture and distance of migration. Results Keratinocyte migration could be significantly promoted by RB, especially at a concentration of 2 × 10－2 mg/mL or 2× 10－3 mg/mL. Compared with controls, keratinocytes migrate in RB medium with a much longer distance and earlier time (4 hours earlier) (P＜0.05), the culture time and migration distance of both RB medium and controls had a linear correlation.Conclusion RB can markedly promote keratinocyte migration, which may play an important role in the early healing of skin wound. 【 Key words】 Single drug prescriptions Rhizoma Bletillae Keratinocytes Organ culture 祖国医学中用于治疗皮肤疮疡溃破的药物甚多，但其作用机制不甚明了。近年来许多研究证明表皮的创伤愈合一般经过角质形成细胞的激活、游走、增生及基底膜的修复等过程，其中角质形成细胞游走在创面覆盖与创伤愈合中起着关键的作用。白芨为止血类中草药，可用于治疗溃疡久不愈合和手足皲裂等症。我们探讨了中药白芨对角质形成细胞游走的影响及其作用机制，现报道如下。 材料与方法 （一）动物来源：实验小鼠系由上海市放射医学研究所提供，上海医学实验动物管理委员会审核合格的红皮鼠品种，SD品系，普通级动物。 （二）动物皮肤样品处理：出生后24～72h的SD小鼠，先以颈折断法致死，再以75％酒精消毒皮肤，经背部切开，游离，取皮。用含