Port-a-Patch數据及应用.doc

Port-a-Patch Data and Applications 窗体顶端 窗体底端 ?hERG通道： hERG通道上的应用： Sticky compounds, such as some hERG blockers, exhibit expected IC50 values with the Port-a-Patch?. The concentrations of the half maximal block were: 1.27 nM (astemizole), 8.9 nM (cisapride), 163.7 nM (flunarizine) and 11.0 nM (terfenadine). Cells were kindly provided by Cytomyx/Millipore, UK. 德国Nanion全自动膜片钳应用：Quinidine对hERG通道的抑制作用 A full dose response curve of quinidine acting on the hERG channel was obtained at physiological temperature (35 °C). The IC50 for quinidine at physiological temperature was 1.3 ± 0.2 μM (n = 5), similar to that obtained at room temperature (1.0 ± 0.03 μM, n = 3). Cells were kindly provided by Cytomyx/Millipore, UK. 德国Nanion全自动膜片钳应用：温度对hERG通道的影响 The image shows example traces for hERG mediated currents at 25 ± 2?°C (black) and 35 ± 2?°C (blue). The peak current amplitude was increased at 35?°C, and the rise time and decay time constants were faster at physiological temperature compared with those obtained at room temperature. Cells were kindly provided by Cytomyx/Millipore, UK. hEAG通道： hEAG (hKv10.1) Block of hEAG whole cell currents after the internal application of 200?μM TEA. Data were kindly provided by Walter Stühmer, MPI for exp. Med., G?ttingen. 德国Nanion全自动膜片钳应用：P2X3通道 Shown are raw current traces from 1321 N1 cells expressing P2X3 receptors demonstrating the reproducibility of the whole cell responses. Currents were activated by 100 ms application of 10?μM ATP. The waiting time between sweeps was 120?s (holding ?60?mV). Cells were kindly supplied by Evotec AG, Hamburg, Germany GABA通道： Ligand dependent activation of GABAA receptors can be recorded with the Laminar Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration dependent activation and desensitization of GABAA (α1β2γ2) receptors was obtained by applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s. 德国Nanion全自动膜片钳应用：在甘氨酸通道 The Laminar Fl