超声波提取杠板归多糖的工艺研究剖析.docx

R917贵州医科大学12届药学专业本科毕业论文超声波提取杠板归多糖的工艺研究学 生： 黄韵蓓学 号： 20120401107年 级： 12 级专 业：药学指导教师：王晓丽教授实习单位：贵州医科大学生药学教研室2016年 4 月 7 日目 录 摘 要 ………………………………………………………… 1 前 言 ………………………………………………………… 2 材料与方法 ………………………………………………… 2 结果与分析 ………………………………………………… 5 讨 论 ……………………………………………………… 10 参考文献 …………………………………………………… 12 致 谢 ……………………………………………………… 13超声波提取杠板归多糖的工艺研究学生：黄韵蓓 导师：王晓丽教授摘要：目的 从杠板归中提取多糖，并测定其含量计算收得率，为杠板归质量控制和资源开发提供科学根据。方法 在单因素考察提取杠板归多糖的基础上，采用正交试验设计法；以杠板归药材多糖含量为指标，用超声波辅助提取，醇沉法得到多糖沉淀，用高浓度醇清洗沉淀，加蒸馏水溶解，采用苯酚—硫酸法在490nm处测定吸光度，以葡萄糖标准品为对照品，计算杠板归多糖的提取率，确定最佳提取工艺。结果 研究3个较显著因素，液料比、提取温度、提取时间对杠板归多糖提取率的影响；在此基础上，用正交试验法确定了杠板归多糖最佳提取工艺为：料液比1:10，提取温度80℃，提取时间30min，最高提取率为3.34%。结论 优化的提取工艺提取多糖效果好，稳定可行，对杠板归多糖的进一步研究和杠板归药材的质量评价具有科学的参考价值。关键词：超声波；多糖；工艺优化Ultrasonic extraction bar plate technology research of polysaccharidesAbstract：Purpose To extract polysaccharide from poles board, and the determination of its content rate calculation, bar plate to provide a scientific basis for quality control, and resource development.Method Polysaccharide extraction bar plate to the single factor investigation, on the basis of orthogonal experiment design method; Herbs polysaccharides content as an index, with bar plate by ultrasonic assisted extraction, alcohol heavy precipitation method for polysaccharide, clean with high concentration of alcohol precipitation, add distilled water to dissolve, using the phenol - sulfuric acid method determination of absorbance at 490 nm, with glucose standard as reference substance, extraction yield of polysaccharide in bar plate to determine the optimal extraction process.ResultResearch three significant factors, liquid ratio, extraction temperature and extraction time on the bar plate to the influence of the polysaccharide extraction yield; On this basis, the poles are determined by using orthogonal test plate for polysaccharide extraction process is: the best material liquid than 1:10, extraction temperature 80 ℃, extracting time 30 min, t