TroponinComplex蛋白分析..docx

Troponin Complex (IC)蛋白分析 一、氨基酸序列：274aa MAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEETSGGGGSGGGGSGGGGSACMDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVEEFLEHHHHHH Troponin I Cardiac (28-110 Region) and Troponin C-Cardiac( full length), 6*His tagged 分析项目 结果 分析工具 基本信息 蛋白名称 心肌钙蛋白Troponin Complex ProtParam HYPERLINK /cgi-bin/protparam/protparam /cgi-bin/protparam/protparam 蛋白序列 MAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEETSGGGGSGGGGSGGGGSACMDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVEEFLEHHHHHH 肽链长度(AA ) 274aa 分子量(kDa) 30592Da 等电点 不稳定指数 pI4.61 The instability index (II) is computed to be 43.03 半胱氨酸数 Troponin I, cardiac muscle 5个Cys,2个二硫键 钙结合位点 两个Ca结合位点分别位于208D212D，244D248D 一级结构 常见氨基酸组成 Ala (A) 18 6.6% Arg (R) 12 4.4% Asn (N) 5 1.8% Asp (D) 26 9.5% Cys (C) 5 1.8% Gln (Q) 11 4.0% Glu (E) 36 13.1% Gly (G) 28 10.2% His (H) 8 2.9% Ile (I) 11 4.0% Leu (L) 27 9.9% Lys (K) 22 8.0% Met (M) 12 4.4% Phe (F) 11 4.0% Pro (P) 4 1.5% Ser (S) 13 4.7% Thr (T) 11 4.0% Trp (W) 0 0.0% Tyr (Y) 4 1.5% Val (V) 10 3.6% ProtParam在线工具， HYPERLINK /protparam/ /protparam/ 属性氨基酸组成 Total number of negatively charged residues (Asp + Glu): 62 Total number of positively charged residues (Arg + Lys): 34 神经网络计算法 Helix Sheet Psipred在线工具 HYPERLINK http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/ 单序列预测方法 10 20 30 40 50 60 70 | | | | | | | MAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDL cccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccchhhhhhchhhhhhhh CRQLHAR