过氧化氢酶和黏附素保守区二价疫苗治疗小鼠幽门螺杆菌感染.docVIP

过氧化氢酶和黏附素保守区二价疫苗治疗小鼠幽门螺杆菌感染 　　　作者：白杨，陈丽，王继德，施理，唱韶红，张兆山，周殿元，张亚历 【关键词】 幽门螺杆菌 Immunzition treatment of Helicobacter pylori infection with two valence vaccine composed of catalase and adhesin conservation: An animal model study 　　【Abstract】 AIM: To investigate the effects of two valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and adhesin conservation in the treatment of H. pylori infection in an H. pylori infected mouse model. METHODS: C57BL/6 mice infected with H. pylori were divided into seven groups. The mice of each group were immunized respectively by catalase and adhesin conservation (separately, 100 μg) plus CT (2 μg), catalase (100 μg) plus CT (2 μg), adhesin conservation (100 μg) plus CT (2 μg), catalase (100 μg), adhesin conservation (100 μg) and CT (2 μg), PBS orally once a week for four weeks. Four weeks after the last treatment, all animals were sacrificed and stomach biopsies were collected to detect H. pylori by semiquantitative bacterial culture assay. RESULTS: The eradication rate of two valence vaccine group, single valence vaccine group of catalase and single valence vaccine group of adhesin conservation was 69.2%（18/26）, 30.8%（8/26） and 38.5%(10/26), respectively. The eradication rate of the other four groups was all 0%. The H. pylori colonized density of the vaccination group was significantly lower than that of the other four groups (Plt;0.05). There were significantly differences in the eradication rate and H. pylori colonized density between the two valence vaccination group and the single valence vaccination groups (Plt;0.05). CONCLUSION: The two valence vaccine consisting of catalase, adhesion conservation and adjuvant has better immunotherapeutic effects than the single valence vaccine. More H. pylori vaccine research should be directed to the multivalence vaccine. 　　【Keywords】 Helicobacter pylori; catalase; adhesin; vaccine 　　【摘要】 目的：　利用人类幽门螺杆菌(H.pylori)感染的小鼠模型研究过氧化氢酶和黏附素保守