黄连、黄芩对3种厌氧菌的体外抑菌活性研究.docVIP

黄连、黄芩对3种厌氧菌的体外抑菌活性研究 【摘要】 目的 研究黄连、黄芩对牙龈紫质单胞菌、中间普氏菌、核梭杆菌厌氧菌的体外抑菌活性，为临床筛选中药治疗厌氧菌感染提供依据。方法 将黄连、黄芩制成煎剂，采用美国临床实验室标准化委员会(NCCLS)推荐的琼脂稀释法，测定此2种药物对3种细菌的最小抑菌浓度（MIC）。结果 黄连对中间普氏菌的MIC为1∶640；对牙龈紫质单胞菌、核梭杆菌的MIC为1∶320。黄芩对牙龈紫质单胞菌、中间普氏菌的MIC为1∶10；对核梭杆菌的MIC为1∶20。结论 黄连、黄芩对上述3种试验菌株均具有不同程度的体外抑菌活性，其中黄连对中间普氏菌的抑菌活性最强，黄芩对3种细菌虽然也显示活性，但均较黄连弱。 【关键词】 黄连；黄芩；琼脂；微生物敏感试验；细菌，厌氧 　　Inhibitory activity of coptidis rhizoma, radix scutellariae on three kinds of anaeroeies in vitro 　　GUO Xiujuan, ZHENG Jinxiu, LI Hong 　　Department of Immmunology, No.4 Hospital of Hebei Medical University, Hebei Province, Shijiazhuang 050011 　　【Abstract】 Objective To investigate the inhibitory activity in vitro of coptidis rhizoma, radix scutellariae on Porphyromonas gingivalis, Provotella intermedons, Fusobacterium nuleartum and provide evidence for drug susceptibility testing of the Chinese herbal. Methods The Chinese herbal was pureed, and adopted the methods of agar dilution recommended by NCCLS to investigate the MIC of Chinese herbal on the three different kinds of bacteria. Results The MIC of coptidis rhizoma on Probotella intermedons was 1∶640, and the MIC on Porphyromonas gingivalis and Fusobacterium nuleartum were 1∶320. the MIC of radix scutellariae on Porphyromonas gingivalis, Provotella intermedons and Fusobacterium nuleartum were 1∶10, 1∶10 and 1∶20 separately. Conclusion The coptidis rhizoma, radix scutellariae had different level inhibitory activity in vitro on the bacteria strain mentioned above. And the coptidis rhizoma had the most intensive inhibitory on Provotella intermedons,the radix scutellariae has lower lever inhibitory activity than the coptidis rhizoma. 　　【Key words】Coptidis rhizoma; Chinese herbal radix scutellariae; Microbe susceptibility testing; bacteria; Anaerobic 　　随着抗生素的大量使用，各种细菌包括厌氧菌的耐药率日趋升高。资料表明，厌氧菌可以通过产酶、细胞膜通透性改变、外排、线粒体突变、耐药基因传递等机制导致耐药率上升，而且长期使用抗生素治疗还可能诱导菌群失调和真菌的二重感染［1～3］。因此，本研究选择了3种致病性厌氧菌牙龈紫质单胞菌、 中间普氏菌、核梭杆菌作为研究对象，采用美国临床实验室标准化委员会(NCCLS)推