黄芪对大鼠酒精性肝病肝组织icam1表达的影响.docVIP

黄芪对大鼠酒精性肝病肝组织ICAM1表达的影响 【摘要】 目的探讨黄芪对大鼠酒精性肝病肝组织ICAM1的影响。方法将36只大鼠随机分为3组（每组12只）：正常对照组（简称对照组）、酒精性肝病模型组（简称模型组）、酒精性肝病黄芪治疗组（简称治疗组）。观察各组肝组织ICAM1的表达及肝组织形态学改变。结果酒精性肝病模型组肝组织ICAM-1的表达明显增强，与正常对照组有显著性差异（P＜0.01）；酒精性肝病黄芪治疗组与酒精性肝病模型组比较肝组织ICAM1的表达明显减弱（P＜0.01），肝组织损害减轻（P＜0.01）。结论 黄芪对酒精性肝病有防治作用，其机理可能与其抑制肝细胞中ICAM1的的表达有关。 　　 【关键词】 酒精性肝病； 细胞间黏附分子-1； 黄芪 　　The Effect of Radis Astragali on the Expression Level of Intercellular Adhesion Molecule-1(ICAM-1) in Liver with Alcoholic Liver Disease in Rats 　Abstract：ObjectiveTo investigate the effect of Radis Astragali on the expression of intercellular adhesion molecule1(ICAM1) in liver with alcoholic liver disease in rats.Methods36 cases of rats were randomly into three groups:control group ,alcoholic liver disease group ,alcoholic liver disease treatment group,12 cases in each.The morphological changes and ICAM-1 expression level in liver were observed.ResultsThe expression level of ICAM1 in alcoholic liver disease group was higher than that in control group(P＜0.01)，the structure of liver tissue was impaired(P＜0.01).The expression level of ICAM1 in alcoholic liver disease treatment group was lower than that in alcoholic liver disease group(P＜0.01),the structure injury in the liver tissue was alleviated (P＜0.01).ConclusionRadis Astragali has satisfactory effect on experimental alcoholic liver disease,the mechanism may be corelated with its inhibition on ICAM1 in liver tissue. 　　Key words：Alcoholic liver disease; Intercellular adhesion molecule1; Radis Astragali 酒精性肝病（Alcoholic liver disease ,ALD）是指长期饮酒引起的酒精性肝损伤，由于发病率呈不断上升趋势，因此我们对酒精性肝病的发病机制及其防治的研究十分重视。近来研究表明，组织细胞间黏附分子1（Intercellurlar adhesion molecule1,ICAM1）与酒精肝病关系密切［1，2］，是引起肝损伤的一个重要因素。本研究采用大鼠酒精性肝病动物模型，观察黄芪对大鼠酒精性肝组织细胞间黏附分子-1表达的影响，为临床上防治酒精性肝病提供理论依据。 　　1 材料与方法 　　1.1 模型制备及动物分组选用健康雄性Wistar 大鼠36只，体重250 g，由咸宁学院动物室提供。随机分为3组，每组12只。正常对照组、酒精性肝病模型组、酒精性肝病黄芪治疗组。采用乙醇浓度、剂量逐渐递增法复制酒精性肝病模型。实验动物正常喂养一周后，除正常对照其余各组均给予40%的乙醇6 g·kg-1·d-1分两次灌胃，以后每2周灌胃乙醇量与浓度逐渐