黄芪对高脂血症小鼠血脂及脂质过氧化的影响.docVIP

黄芪对高脂血症小鼠血脂及脂质过氧化的影响 【摘要】 目的探讨黄芪对高脂血症小鼠血脂及脂质过氧化的影响。方法40只小鼠随机分为5组：正常对照组、高脂血症组和不同剂量黄芪组，除正常组外，其他组的小鼠灌服脂肪乳剂建立小鼠高脂血症模型，黄芪按18，9 ，4.5 g/kg 剂量给小鼠连续灌胃4周后，测定血清总胆固醇（TC），甘油三酯（TG），高密度脂蛋白胆固醇（HDL-c）、低密度脂蛋白胆固醇（LDL-c），谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）活性及脂质过氧化物（LPO）。结果黄芪能明显降低TC、TG和LDL-c的浓度（Plt;0.05），能明显升高血SOD和GSH-Px活性（Plt;0.05），降低血清LPO的浓度（Plt;0.05）。 结论黄芪能调节小鼠高脂血症脂代谢和增强抗脂质过氧化的作用。 【关键词】 黄芪； 高脂血症； 血脂； 脂质过氧化 　　Effects of Astragalus on Serum Lipids and Lipoperoxidation in Experimental Hyper Lipidemic Mice 　　　Abstract：ObjectiveTo investigate the effects of Astragalus on serum lipids and lipoperoxidation in experimental hyperlipidemic mice. Methods40 mice were randomly divided into normal group, hyperlipidemia control group, experimental control groups. Apart from the mice in normal group, all the mice in the other groups were fed with lipid emulsion for 4 weeks. After the experimental hyperlipidemic mice were succeeded, experimental control group1, 2 and 3 in which the mice were treated with Astragalus at dosages of 18, 9, 4.5 g/kg respectively. After 4 weeks, the TC, TG, HDL-c, LDL-c, SOD, GSH-Px and LPO in serum were measured.ResultsAstragalus could significantly decrease the serum TC, TG, HDL-c in the experimental hyperlipidemic mice, and markedly increase the level of serum HDL-c. Mean level of serum LPO in the experimental groups treated by Astragalus at different dosages were much lower than that in hyperlipidemia group, and the SOD and GSH-Px activities of serum in the group of Astragalus were much higher than those in hyperlipidemia group. ConclusionAstragalus can regulate lipid metabolism, enhance the antioxidation and reduce the lipid peroxidation in mice with hyperlipidemia. 　　Key words：Astragalus; Lipoperoxidation; Serum lipids； Lipoperoxidation 　　黄芪具有补气升阳、固表止汗、托疮生肌、利水消肿之功效，用于治疗表虚自汗、阳虚血脱及水肿血痹等症。本实验研究黄芪水提物对高脂血症小鼠体内血脂及脂质过氧化的影响。 　　1 材料 　　1.1 药物与试剂丙基硫氧嘧啶，上海复兴朝辉药业有限公司产品；胆固醇，去氧胆酸钠，猪油，自制。GSH-Px，SOD，LPO试剂盒，均为南京建成生物工程研究所产品。其他试剂均为国产分析纯。 　　1