黄芩茎叶总黄酮对原代培养大鼠肝细胞损伤的保护作用.docVIP

黄芩茎叶总黄酮对原代培养大鼠肝细胞损伤的保护作用 作者：李素婷，杨鹤梅，周晓慧 【关键词】 黄芩茎叶总黄酮；,,肝细胞；,,原代培养；,,保护作用；,,机制 　　摘要：目的研究黄芩茎叶总黄酮（SSTF）对原代培养大鼠肝细胞氧化损伤的保护作用及其机理。方法采用胰蛋白酶消化、分离大鼠肝细胞进行原代培养，用H2O2和CCl4体外诱导肝细胞损伤，同时加以不同浓度的SSTF对肝细胞保护，检测培养液中丙氨酸氨基转移酶（ALT）活性、肝细胞内丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSHpx）活性，MTT法测定肝细胞增殖活性。结果与模型组比较，SSTF明显抑制H2O2和CCl4引起的肝细胞培养液中ALT活性的升高，肝细胞存活率明显升高，还可明显恢复H2O2引起的肝细胞MDA含量的升高和GSHpx 活性的降低明显。结论 SSTF对培养大鼠肝细胞损伤有明显的保护作用，其机制可能与其抗脂质氧化作用有关。 　　关键词：黄芩茎叶总黄酮； 肝细胞； 原代培养； 保护作用； 机制 　　Study on the Protective Effect and Possible Mechanism of Scutellaria baicalensis Stem-leaf Total Flavonoid on Primary Cultured Rat Hepatocytes Injured by H2O2 or CCl4 　　Abstract：ObjectiveTo study the protective effect and possible mechanism of Scutellaria baicalensis stem-leaf total flavonoid(SSTF) on primary rat hepatocyes injured by H2O2 or CCl4.MethodsPrimary hepatocytes were isolated,cultured by trypsin digestion and induced by H2O2 or CCl4.Various concentrations of SSTF were added to the primary cultured hepatocytes .The contents of malondialdehyde(MDA) and activity of glutathion peroxidase(GSH-px) in hepatocytes and the activity of ALT in cultural supernatant were determined by general methods,the viabilities of hepatocytes were measured by MTT. ResultsCompared with model group, SSTF could restore remarkably ALT level in supermatant of cultural hepatocytes, decrease MDA content and elevate GSHpx activity of hepatocytes ,hepatocyte viabilities increased significantly by SSTF.ConclusionSSTF has a protective action on primary rat hepatocyes injured by H2O2 or CCl4. These might be associated with its antilipoperoxidation function. 　　Key words：SSTF; Hepatocytes ; Primary cultured ; Protective effect; Mechanism. 　　黄芩茎叶总黄酮（Scutellaria baicalensis stem-leaf total flavonoid ，SSTF）是承德医学院中药研究所从唇型科植物黄芩的茎叶部分提取的有效成分，整体实验已证实SSTF具有抗炎、抗氧化、抗动脉粥样硬化及保肝等作用［1～4］。为进一步研究SSTF保肝作用及其机制，本文采用胰蛋白酶消化法分离肝实质细胞进行体外培养，用CCl4和 H2O2体外诱导肝细胞氧化损伤模型，在细胞水平上证实SSTF对肝细胞损伤的保护作用及其机制，为进一步阐明SSTF的药理作用及中药新药的研发和应用提供可靠依据。 　　1 材料与方法 　　1