甲醛对小鼠生精细胞bcl2、bax蛋白表达及超微结构改变的影响.docVIP

甲醛对小鼠生精细胞Bcl2、Bax蛋白表达及超微结构改变的影响 【摘要】 目的:研究甲醛对小鼠睾丸生精细胞Bcl-2、Bax蛋白表达及超微结构的改变。方法:取24只雄性清洁级（ICR）小鼠，随机分为4组，每组6只，用0.2、2、20 mg/kg甲醛腹腔注射，每天一次，连续5 d，第6天用免疫组化法测定小鼠睾丸生精细胞的Bcl-2、Bax蛋白表达率；用电镜观察小鼠睾丸生精细胞超微结构变化。结果:小鼠睾丸生精细胞的Bcl-2蛋白表达率明显低于对照组，差异有显著性（P＜0.05），Bax蛋白表达率明显高于对照组，差异有显著性（P＜0.05）；电镜观察结果显示，各浓度甲醛能使生精细胞的细胞核、染色质、线粒体等超微结构发生不同程度的病理改变。结论:甲醛可使小鼠生精细胞Bcl-2和Bax蛋白表达及超微结构发生显著性改变，从而诱导细胞凋亡。 【关键词】 甲醛 Bcl-2 Bax 生精细胞 Abstract: Objective:To study the effects of formaldehyde on Bcl-2, Bax expression in spermatogenic cell of mice and ultrastructural changes in spermatogenic cell. Methods: Twenty-four male mice (ICR) were divided into 4 groups randomly, who were treated with formaldehyde of 0.2、2、20 mg/kg respectively one time a day, and lasted for 5 days, the Bcl-2, Bax protein expression rate was tested in the sixth day with the immunohistochemical method; and the ultrastructural changes of spermatogenic cell and sperm were observed under the transmission electron microscope. Results: Bcl-2 protein expression rates were lower than that of the contrast group, the difference had statistical significance（Plt;0.05）. Bax protein expression rate was higher than that of the contrast group, the difference had statistical significance（Plt;0.05）. The result under electron microscope showed that the ultrastructure of karyon, karyotin, mitochondria and karyotheca in each treated group had different degree of damage by serving formaldehyde in different concentrations.Conclusion: Formaldehyde exposure will cause the remarkable changes of Bcl-2, Bax protein expression, and ultrastructural changes in spermatogenic cell and sperm, which led to cellular apoptosis. Key words: formaldehyde, Bcl-2, Bax, spermatogenic cell 动物实验发现,甲醛具有生殖毒性和致细胞突变及致癌性[1]。对甲醛毒性研究一般集中在器官和组织水平，从分子水平探讨甲醛对机体损伤及细胞凋亡的研究国内较少。为了进一步研究甲醛睾丸细胞毒性和生殖细胞的调亡机制，本研究分析Bcl-2、Bax蛋白表达及小鼠睾丸生精细胞超微结构变化情况,报告如下。 1 材料和方法 1.1 主要试剂 AR甲醛（Formaldehyde，FA），由上海溶剂厂提供，Bal-2、Bax免疫组化试剂盒（购自武汉博士德生物制剂有限公司），戊二醛（购自中国医药集团上海化学