甲型与乙型流感病毒诱导肿瘤细胞凋亡的比较.docVIP

甲型与乙型流感病毒诱导肿瘤细胞凋亡的比较 作者：桂芳，张卓然，段于峰，周志航，滕兰菊，李争鸣 【摘要】 目的观察并比较不同流感病毒诱导肿瘤细胞凋亡能力的差异。方法A1、 B型流感病毒及经紫外线照射后的病毒分别感染HeLa细胞，于不同时间取HeLa细胞分别在电镜下观察细胞的形态，Annexin VFITC染色流式细胞仪检测凋亡百分率。结果A1亚型及B型流感病毒作用于HeLa细胞后24h，电镜下可见细胞凋亡的特征性改变，且凋亡百分率随时间延长而增高，于12~24h达高峰，其中B型流感病毒的凋亡诱导率最高达38.65%，A1亚型最高为23.94%。紫外线照射后的B型流感病毒（UVB）最高达到18.20%，UVA1最高达到10.05%，A1亚型与B型相比差异有统计学意义（Plt;0.01）。结论B型流感病毒诱导肿瘤细胞凋亡的能力比A型流感病毒强，而且经紫外线照射后的流感病毒可能仍然具有一定的诱导细胞凋亡能力；在肿瘤治疗方面，B型流感病毒比A型流感病毒具有更大的研究和应用价值。 【关键词】 流感病毒；肿瘤细胞；细胞凋亡 　　Abstract：Objective To observe and compare the difference of apoptosis in tumer cells induced by different influenza viruses. MethodsThe shapes of HeLa cells in different time were observed in the electron microscope after they were infected by influenza virus type A1, B and ultraviolet exposure virus respectively. We used Annexin VFITC staining flow cytometry (FCM) to observe the percentage of apoptosis. ResultsTypical apoptosis in HeLa cells were observed in microscope after infected by influenza viruses type A1 and B 24 hours. The percentage of apoptosis increased along with time expanded and culminated from 12 to 24 hours. The maxima of the percentage of apoptosis induced by influenza virus type B, A1, ultraviolet exposure influenza B virus(UVB) and ultraviolet exposure influenza A1 virus (UVA1) come to 38.65%,23.94%, 18.20% and 10.05% respectively. There have significant difference between influenza A1 and B virus (Plt;0.01). ConclusionInfluenza B virus is more strong than influenza A virus in the ability of inducing apoptosis. More over, the ultraviolet exposure influenza virus perhaps still has the certain ability of inducing apoptosis in HeLa cells. Compared with the influenza A virus, influenza B virus have greater research and application value in the treatment of cancer. 　　Key words：Influenza virus；Tumer cell；Apoptosis 　　0引言 　　细胞凋亡是细胞在生理或病理信号刺激下，启动自身凋亡相关基因而发生的主动“自杀”过程。近年来的许多研究表明，流感病毒可诱导多种细胞发生细胞凋亡，包括其容纳细胞和非容纳细胞，特别是A型流感病毒研究较多。由于流感病毒可诱导多种肿瘤细胞发生细胞凋亡，