碱性成纤维细胞因子对真皮下血管网皮瓣成活影响的实验研究.docVIP

碱性成纤维细胞因子对真皮下血管网皮瓣成活影响的实验研究 作者：尹刚 贾堂宏 刘志功 孙炳卫 龚维明 刘淑恒 郭淑亚 张庆国 【摘要】目的：研究碱性成纤维细胞生长因子（bFGF）对促进随意型真皮下血管网皮瓣（SVNF）成活的作用。方法：分别在4只纯白色家猪两侧背部各形成6个SVNF，长16cm，宽4cm，将24个皮瓣随机分为bFGF组和对照组，于术后6d测量各组皮瓣成活率及皮瓣新生血管密度。结果：术后6d，bFGF组与对照组SVNF成活率分别为（81.2±1.7）%和（66.2±1.8）%(P＜0.05)，皮瓣近、中、远段皮瓣血管密度分别为（16.7±6.0）、(29.7±5.5)、（14.9±6.0）/mm2和（16.6±4.0）、（21.4±5.2）、（3.5±5.1）/mm2(P＜0.05)。大体观察和组织学观察bFGF组均优于对照组。结论：皮瓣下局部注射bFGF后，通过促进SVNF远端基底部和边缘部的血运，可以提高SVNF成活率，缩短皮瓣的断蒂时间，扩大皮瓣长宽比例。 【关键词】真皮#12539;外科皮瓣#12539;成纤维细胞生长因子2 BFGF improves the viability of subdermal vascular network flap 【ABSTRACT】Objective:To investigate the application valve of bFGF to improve the viability of subdermal vascular network flap.Methods:Four white healthy pigs were used,each pig had 6 SVNF on back,16cm×4cm，totally 24 flaps were divided randomly into 2 groups:The bFGF groups and the control groups.On postoperative day 6,we observed the appearance of the flaps.The survival area of each flap was measured and the flap was harvested for histological analysis and the density of angiogenesis was also examined.Results:On postoperative day 6,the survival rates of the bFGF group and the control group were (81.2?.7)% and (66.2?.8)% respectively,the angiogenesis density of the middle and the distal segment in the bFGF group was (16.7?.0)/mm2,(29.7?.5)/mm2,(14.9?.0)/mm2 respectively,while (16.6?.0)/mm2,(21.4?.2)/mm2,(3.5?.1)/mm2 respectively in the control group.The bFGF group was superior to the control group in the appearance and histological analysis of the flaps.Conclusion:The results suggest that the injection of bFGF locally can promote the survival rate by accelerating the blood circulation of the SVNF?bottom and edge,shorten the division time of SVNF and broaden the ratio of length and width. 【KEY WORDS】Dermis#12539;Surgical flap#12539;Fibroblast growth factor 2 　 临床上经常见皮肤撕脱伤、热压伤、严重烧伤创面，早期良好的组织覆盖是外科修复的重要研究内容，对恢复患肢功能，保留良好的外观至关重要。随意型真皮下血管网皮瓣（subdermal vascular network flap,SVNF）是近20年