胶束电动毛细管色谱法测定半夏等药材中苯甲酸的含量.docVIP

胶束电动毛细管色谱法测定半夏等药材中苯甲酸的含量 作者：曹艳， 黄必胜， 王平 ， 陈科力 【关键词】 半夏；,,掌叶半夏；,,水半夏；,,苯甲酸,；,,胶束电动毛细管色谱法 　　摘要：目的建立胶束电动毛细管电泳色谱法(MECC)测定半夏及其同科其它品种药材中苯甲酸的含量的方法。方法用MECC法对样品进行分析。毛细管柱内径75 μm，长62.5 cm；运行电压25 kV，检测波长200 nm，分离温度35℃；冲液为30 mmol#12539;L1，SDS30 mmol#12539;L1硼酸15％甲醇（pH值9.5）；压力进样，进样时间3 s。结果苯甲酸在0.044～2.2 mg/ml浓度范围内具有良好的线形关系（ r＝0.9994）， 平均回收率为96.7% (RSD=2.4%, n=6), 对半夏及其同科属其它品种共14个样品中的苯甲酸含量进行了测定。结论该方法快速、简便、并且较为准确，可供评价半夏等品种质量时参考。 　　关键词：半夏； 掌叶半夏； 水半夏； 苯甲酸 ； 胶束电动毛细管色谱法 　　Abstract：ObjectiveThe method of micellar electrokinetic capillary chromatography（MECC）was established to determine the contents of benzoic acid in Rhizoma Pinelliae and several Rhizomes from other Araceae species; MethodsThe samples from rhizomes of P. ternana and other species of Araceae were analyzed by MECC; The Capillary column was 62. 5 cm long and 75 μm in internal diameter; The operation voltage 25 kV ; test wavelength 200 nm; column temperature 35 ℃; the mobile buffer was 30 mmol#12539;L1 SDS30mmol#12539;L1 boric acid 15% methyl alcohol (pH 9. 5); The sampling was conducted by pressure, the sampling time was 3 seconds. ResultsThe linear range of determination for benzoic acid was 0. 044~2.2 mg/ml (r =0. 999 4). The average recovery rate was 96.7%(cv =2.4%,n =6). The contents of benzoic acid in the 14 samples from P. ternana and other species of Araceae were determined. ConclusionThe method is fast, simple, convenient, comparatively accurate, and can be used to refer when appraising quality of Rhizoma Pinelliae and several species. 　　Key words：Pinellia ternana; Pinellia pedatisecta; Typhonium flagelliforme; Benzoic acid; MECC 　　半夏为天南星科植物半夏Pinellia. ternata (Thunb.) Breit.的块茎，是常用中药，具有燥湿化痰、降逆止呕、消痞散结的功效［1］。半夏含有苷类、酚类、甾醇类、生物碱类以及有机酸如琥珀酸、苯甲酸等成分。半夏中的有机酸是活性部位之一，何凌云等［2］曾用薄层扫描法对半夏药材中琥珀酸的含量进行了测定；我们也曾采用HPLC法对半夏中琥珀酸进行含量测定（《中国医院药学杂志》待发表）。毛细管电泳法是一种灵敏度较高的含量测定法，本文探索应用MECC法对半夏中所含另一种有机酸苯甲酸进行含量测定。天南星科中有一些中药材为半夏的易混品，如掌叶半夏、水半夏等与半夏的形态、结构、功效较为类似，均含有琥珀酸、苯甲酸等成分，因而也一并进行测定。苯甲酸的含量测定已有多种方法报道，