结直肠癌中cks1, p27kip1和skp2蛋白的表达.docVIP

结直肠癌中Cks1, P27Kip1和Skp2蛋白的表达 作者：赵勇，高建飞，章必成，杜光祖 【关键词】 结直肠癌 　　Cks1, P27Kip1 and Skp2 protein expression in colorectal cancer 　　【Abstract】 AIM: To investigate the expression of Cks1 protein and its relationship with the expressions of Skp2, P27Kip1 and colorectal tumorigenesis. METHODS: Flow cytometry was used to detect the expressions of Cks1, Skp2 and P27Kip1 in 20 normal colorectal mucosas, 20 adenomas as well as 54 colorectal cancers. RESULTS: The expressions of Cks1 and Skp2 gradually increased in the order of normal mucosa, adenoma to colorectal cancer (Plt;0.05), while the expression of P27Kip1 gradually decreased in the same order (Plt;0.05). A strong correlation was found between the expressions of Cks1 and Skp2 (r=0.845, Plt;0.05) and a significant inverse relation was also observed between the expressions of Cks1 or Skp2 and of P27Kip1 (r=-0.752, Plt;0.05; r=-0.746, Plt;0.05 respectively). The expression of Cks1 was significantly associated with the loss of tumor differentiation (Plt;0.05), but not with lymph node metastasis and Dukes staging (Pgt;0.05). CONCLUSION: Cks1 may play an important role in human colorectal tumorigenesis. Cks1 may be an essential factor in proteasomedependent degradation of P27Kip1 regulated by Skp2. 　　【Keywords】 colorectal neoplasms; cyclin kinase subunit 1; S phase kinase protein 2; P27Kip1 protein; ubiquitin 　　【摘要】 目的： 探讨Cks1在结直肠癌发生及其在Skp2调节P27Kip1降解过程中的作用. 方法： 应用流式细胞术测定正常结直肠黏膜、结直肠腺瘤和结直肠癌组织中Cks1, P27Kip1和Skp2蛋白的表达. 结果： 由正常结直肠黏膜、结直肠腺瘤到结直肠癌Cks1, Skp2表达呈上升趋势(P＜0.05)，P27Kip1表达呈下降趋势(P＜0.05). 结直肠癌中Cks1, Skp2表达与P27Kip1表达呈负相关(r=-0.752, P＜0.05; r=-0.746, P＜0.05)；Cks1与Skp2呈正相关（r=0.845, P＜0.05）. 结直肠癌中Cks1的表达与肿瘤分化程度相关（P＜0.05），而与淋巴结转移及Dukes分期不相关（P＞0.05）. 结论： Cks1可能参与了结直肠癌的发生；结直肠癌中Cks1可能参与了Skp2调节P27Kip1泛素化降解过程. 　　【关键词】 结直肠肿瘤；Cks1蛋白；Skp2蛋白；P27Kip1蛋白；泛素 　　0引言 　　许多恶性肿瘤中细胞周期性蛋白P27Kip1的表达是降低的，Fbox蛋白S phase kinase protein 2 （Skp2)参与了P27Kip1泛素化降解过程. Cyclin kinase subunit 1 （Cks1)是高度保守的细胞周期调节蛋白Suc1