中医论文开题报告.doc

湖北中医药大学2013级硕士生 开题报告 百合知母汤抗抑郁细胞内信号转导通路的机制研究 研 究 生 姓 名：*** 导师姓名、职称：**** 教授 学 科 专 业：基础医学院 研 究 方 向：方剂的配伍规律及临床运用 2015年4月目录 一、研究目的与意义·········································3 1、研究目的··············································3 2、研究意义··············································3 二、研究现状·············································3 1、百合病的研究现状········································3 2、抑郁症的研究现状········································4 3、百合知母汤的研究现状·····································6 三、研究思路与立题依据·····································6 1、研究思路··············································6 2、立题依据··············································7 四、研究内容及目标·········································· 7 五、拟解决的关键问题········································7 六、研究方案·············································8 1、实验动物··············································8 2、药物制备··············································8 3、CUMS抑郁症模型大鼠制备···································· 8 4、行为学指标的测量········································9 5、尼氏染色··············································10 6、生化指标的检测··········································10 七、技术路线图·············································12 八、可行性分析·············································12 九、课题创新性·············································13 十、预期结果·············································13 十一、课题经费预算·········································13 参考文献·················································14 一 研究目的与意义 1.研究目的 百合病最早见于张仲景的《金匮要略》，属中医情志病范畴。《金匮﹒百合狐惑阴属阳毒病证治》篇将“百合病”描述为：“意欲食复不能食，常默然，欲卧不能卧，欲行不行，饮食或有美时，或有不用闻食臭时，如寒无寒，如热无热，口苦，小便赤，诸药不能治，得药则剧吐利，如有神灵者，身形如和，其脉微数。”从百合病的临床表现看，与抑郁症的诊断标准有较多的相似之处。所以现代不少医家都将中医的百合病归属于抑郁症[1,2,3]。百合知母汤为治疗百合病的经典方药之一，由百合和知母两味药组成。百合宁心安神，润肺止咳；知母清热泻火，滋阴润燥。百合甘寒清润而不腻，知母苦寒降火而不燥。百合偏于补，知母偏于泻。二药伍用，一润一清，一补一泻，共奏润肺清热，宁心安神之效。主治阴虚或温热病后余热末清，以致头昏、心烦不安、失眠等症以及情志不遂，以致精神恍惚、不能自制等症。 故本课题旨在通过体动物实验揭示百合知母汤抗抑郁可能的细胞内信号转导机制。 2.研究意义 抑郁症是常见的危害人类身心健康一类精神疾病，以显著而持久的情感或心境低落为主要特征，在WHO的一