甘肃地区鸡肉中四环素类药物残留量的分析研究(定稿).doc

本科学位论文 甘肃地区鸡肉中四环素类药物残留量的分析研究 学院：生命科学与工程学院 专业：09级食品科学与工程（1）班 姓名：褚宝磊 学号：P092114328 2013年4月27日 甘肃地区鸡肉中四环素类药物残留量的分析研究 专业：食品科学与工程 姓名：褚宝磊 指导教师：穆晓峰 摘 要 四环素类药物其中包括土霉素、四环素和金霉素等，是以四并苯为母核的一族抗生素，被广泛用于防治动物肠道感染和促进畜禽生长, 提高成活率和饲料转化率，但由于添加的数量、方式和方法不当, 如持续使用使病原菌容易诱导耐药菌株, 损害肾功能和造血系统, 为此我国对动物性食品中的抗生素药物残留进行了规定[1]。同时也制定了相应的国家标准，但是目前对于一些散养鸡的监管还不到位，为此本实验的主要目的就是对甘肃省部分地区的肉鸡中的四环素类药物进行检测，以确保消费者食用鸡肉的安全性。 实验组从兰州市张苏滩批发市场采集不同地区鸡肉样品24份，该样品分别来自：永登、景泰、靖远、榆中、临夏、武威。将活鸡现宰后取鸡胸部肌肉，采用酶联免疫法观察这6个地区鸡肉中四环素残留情况，并进行逐步研究甘肃省各个不同地区鸡肉中四环素类药物残留量所存在差异性的原因。 关键词 四环素，鸡肉，ELISA试剂盒，残留量，检测 ABSTRACT Tetracyclines oxytetracycline, tetracycline and chlortetracycline, and benzene as the nucleus of a family of antibiotics are widely used to control the animal intestinal infection and promote animal growth, improve the survival rate and feed conversion ratio. However, due to improper add a number of ways and means, such as the continued use of easy pathogens resistant strains induced damage to the renal and hematopoietic system, this country the antibiotic drug residues in animal food, but also developed a corresponding national standards, but is not yet in place for the supervision of some scattered chicken, this experiment in Gansu Province, parts of broiler tetracyclines detection. Key Words: Tetracycline Chicken ELISA kit Residues Detect 1. 引 言················································ 1 1.1 土霉素的理化性质·····································3 1.2四环素类药物的药理作用及毒理作用·····················4 1.3.四环素类药物在动物生产中的应用····················· 4 1.4 我国四环素类药物非法使用的综合治理情况············· 5 1.5 四环素类药物 的检测方法·····························5 1.5.1酶联免疫一步法检测方法（ELISA）····················6 1.5.2高效液相色谱-串联质谱法（LC-MS/MS）················6 1.5.3固相萃取-高效液相色谱法(SPE-HPLC)················6 1.5.4反向高效液相色谱法（RP-HPLC） ····················7 2.材料与方法··········································· 7 2.1 材料··············································· 7 2.1.1试剂···············································7 2.1.2仪器