Dissecting Lepidoptera genitalia Course Information对鳞翅目昆虫的外生殖器课程信息.ppt

Lepidoptera genitalia Preparing female genitalia prepartion is very simple since structures are mounted essentially ‘as is’; ie little manipulation needed most important to be familiar with position and location of structures * SWRS Lepidoptera Course, Aug. 2012 Chris Schmidt Canadian National Collection of Insects Ottawa, CAN Tools for identification and taxonomy: morphology (genitalia, antennae, other external) morphometrics DNA immature stages Morphology - genitalia usually most important character, but numerous other character sets: Antennal morphology Immature stages Biological (eg. flight time, behaviour, host plant use, etc.) Morphometrics Morphology cannot always discriminate sibling species (i.e. overlap of characters between species): how to deal with species complexes? - Quantify multiple character sets and do statistical comparison – morphometrics eg. digitally analyze colour components of wing patterns Morphometrics e.g. spruce budworm complex: (Lumley Sperling 2010 Syst. Ent.) - in this case, greater discriminant ability than DNA barcode Genitalic morphology: very important character set in Lepidoptera taxonomy. - genitalia structurally complex (so lots of characters) - relatively little within-species variation - high between-species variation Genitalic morphology late 1800’s: JB Smith - snapped off the valves of noctuids eg. Smith Dyar 1899: Acronycta revision early 1900s: Hampson - “Catalogue of the Lepidoptera Phalaenae” remains one of the most important works in “noctuidology” – but did not use genitalia (retired about 1912) History of genitalic morphology ~1910: Pierce – “Genitalia of the British Lepidoptera” - also included female genitalia 1900-1920s: Barnes McDunnough (“Contributions...”) - looked at and used genitalia, although not often illus. (later volumes); McD started at CNC in 1921, extensive dissections thereafter Pierce 1914 1950: DF Hardwick - techniques paper the first to describe genitalic dissection and inflati