生长激素促进体外培养的Bel7402肝癌细胞增殖.doc

生长激素促进体外培养的Ｂｅｌ-７４０２肝癌细胞增殖 　　作者：刘建平， 陈涛， 陈小萱， 区庆嘉 作者单位：(中山大学附属第二医院普外科，广东 广州510120) 　　【摘要】 【目的】 了解重组人生长激素(rhGH)对体外培养的Bel-7402肝癌细胞增殖的影响。【方法】 应用放射性配体法了解GHR在Bel-7402肝癌细胞株的表达情况;采用肿瘤细胞计数、噻唑蓝比色法和集落形成实验了解Bel-7402肝癌细胞株在不同浓度rhGH(0、1、10、100、1 000、10 000 ng/mL)作用下的药物敏感性，计算细胞生长率，集落形成率;并用3H-TdR渗入法了解肿瘤细胞DNA代谢情况。【结果】 放射配体法发现Bel-7402肝癌细胞表达GHR， rhGH对体外培养的7402肝癌细胞的生长具有一定程度的刺激作用，表现为rhGH在100 ng/mL的浓度时促进肝癌细胞的增殖较为显著，而rhGH在其它浓度时(1、10、1 000、10 000 ng/mL)的部分时段，对肝癌细胞的增殖虽也能表现出一定程度的促进作用，但总体效果较rhGH 在100 ng/mL浓度时为弱。【结论】 一定浓度范围的rhGH对7402肝癌细胞的增殖有促进作用，原因可能与7402肝癌细胞表达GHR有关。 　　【关键词】 重组人生长激素; 生长激素受体; 原发性肝癌; 细胞培养; 放射配体分析法 　　RhGH Increase Proliferation of Human Hepatic Carcinoma Cell　Line Bel-7402 in Vitro 　　LIU Jian-ping， CHEN Tao， CHEN Xiao-xuan， OU Qing-jia 　　( Department of General Surgery， The Second Affiliated Hospital， SUN Yat-sen University， Guangzhou 510120， China ) 　　Abstract： 【Objective】 To study the effect of rhGH with different concentrations on the growth of Bel-7402 human hepatic carcinoma cell lines in vitro. 【Methods】 Radioligand assay was used to detect the growth hormone receptor (GHR) expression of the hepatic carcinoma cell lines. Tumor cell count， thiazole blue chromatometry， and colony forming test were introduced to determine the sensibility of Bel-7402 to the different concentrations of rhGH (0， 1， 10， 100， 1 000， and 10 000 ng/mL). Growth rate of tumor cells was calculated. Metabolism of DNA of tumor cells was determined by the method of mixture of 3H-TdR. 【Results】 Radioligand assays showed GHR in Bel-7402 hepatic carcinoma cell strain. The rhGH had certain effects of the growth of 7402 hepatic carcinoma cell line. It showed positive effect to accelerate the proliferation on the growth of the hepatic carcinoma cells when the concentration of rhGH reached 100 ng/mL(P 0.05，compared with control group). Although other rhGH concentrations (1， 10， 1 000， and 10 000 ng/mL)also had positive effects， they were much weaker than that at 100 ng/mL 24 hours after