腺病毒介导的血管内皮生长因子165对扩张型心肌病大鼠心功能的影响.doc

腺病毒介导的血管内皮生长因子165对扩张型心肌病大鼠心功能的影响 　　作者：肖俊会1,2， 曾秋棠2， 张宏考1， 王家宁1* 作者单位：(1郧阳医学院附属人民医院心血管内科，湖北 十堰 442000;2华中科技大学同济医学院附属协和医院心血管病研究所，湖北 武汉 430022) 　　【摘要】 目的:观察经心包腔注射腺病毒介导的血管内皮生长因子165基因(Ad-VEGF 165)对扩张型心肌病(DCM)大鼠心功能的影响并探讨其作用机制。方法：35只雌性SD大鼠腹腔注射多柔比星建立扩张型心肌病模型，随机分为2组：VEGF组(18只)心包腔内注射0.1 mL Ad-VEGF 165 1.0×109 pfu;DCM组(17只)注射生理盐水。比较4周后大鼠左室收缩末期容积(LVES)、舒张末期容积(LVED)、左室收缩末期内径(SD)、左室舒张末期内径(DD)、左室内径缩短率(FS)、左室射血分数(LVEF)和体重(BW)、心率(HR)的变化，观察大鼠心肌组织改变情况，ELISA法测定血清APO-1/Fas含量。结果：VEGF组BW、LVEF、HR、FS较DCM组明显增加(P0.01);LVES、LVED、SD、 DD均明显降低(P0.05)，APO-1/Fas水平较DCM组下降(P0.01);注射VEGF后大鼠BW、LVEF、HR、FS较注射前明显增加(P0.01);而LVES、LVED、SD、 DD则明显降低(P0.05)，大鼠的心肌病变减轻。结论：心包注射Ad-VEGF165基因可改善DCM鼠心功能，降低其死亡率，有利于心肌细胞的存活，其机制可能与减少心肌细胞凋亡有关。 　　【关键词】 血管内皮生长因子;扩张型心肌病;超声心动图 　　e-mail:rywjn@Effects of Adenovirus-mediated Vascular Endothelial Growth Factor 165 Gene on Cardiac Function in SD Rats of Dilated Cardiomyopathy Induced by Adriamycin via Pericardial Cavity XIAO Jun-hui1,2,ZENG Qiu-tang2,ZHANG Hong-kao1,WANG Jia-ning1* (1Department of Cardiology,Renmin Hospital,Yunyang Medical College,Shiyan,Hubei 442000;2Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430022,China) 　　Abstract: Objective To study the effects of adenovirus-mediated vascular endothelial growth factor 165 gene on cardiac function in SD rat model of dilated cardiomyopathy(DCM) induced by adriamycin injection via pericardial cavity and explore its possible mechanism.Methods SD rat model of DCM was established at a dose of 2mg/kg once a week by adriamycin intraperitoneally injected and were randomly divided into experimental group and control group.Rats in experimental group received 1.0×109 pfu Ad-VEGF 165 injections via pericardial cavity(VEGF group),while 0.9 % sodium choloride was injected into rats in control group(DCM group).The pericardium was pretreated by injecting a mixture of collagenase(250U)and hyaluronidase(600U).In order to evaluate the effects of Ad-VEG