苦参山楂合剂对单侧输尿管梗阻大鼠结缔组织生长因子表达的影响.doc

苦参山楂合剂对单侧输尿管梗阻大鼠结缔组织生长因子表达的影响 　　作者：姚钢炼　廖婷婷　宁宁　高登峰　桂保松 作者单位：西安交通大学医学院第二附属医院肾病科，陕西西安 710004 　　【摘要】 目的 探讨中药复方苦参山楂合剂对肾间质纤维化大鼠结缔组织生长因子(CTGF)表达的影响以及其对肾间质纤维化的防治作用。方法 40只雄性SD大鼠,随机分为假手术组、模型组、苯那普利组、苦参山楂合剂小剂量组和大剂量组。以单侧输尿管结扎术建立肾间质纤维化的动物模型。术后第14天取术侧肾组织做HE、Masson染色及CTGF、Α平滑肌动蛋白(ΑSMA)和Ⅲ型胶原蛋白(ColⅢ)免疫组化检测，并运用图像分析系统作半定量分析。结果 苦参山楂合剂可以显著改善梗阻侧肾组织病理变化，下调CTGF表达，减少ΑSMA及ColⅢ沉积。结论 苦参山楂合剂可能通过下调CTGF并抑制肌成纤维细胞的活化来起到防治梗阻性大鼠肾间质纤维化的作用。 　　【关键词】 苦参山楂合剂;纤维化;肾间质;结缔组织生长因子;肌成纤维细胞 　　Effect of matrine and crataegus mixture on connective tissue　growth factor in unilateral ureteral obstruction rats 　　Yao Ganglian, Liao Tingting, Ning Ning, Gao Dengfeng, Gui Baosong 　　(Department of Nephrology, the Second Affiliated Hospital,Medical School of Xian Jiaotong University, Xian 710004, China) 　　ABSTRACT: Objective To investigate the protective effect of matrine and crataegus mixture on the renal interstitial fibrosis in unilateral ureteral obstruction (UUO) rats. Methods Forty male SpragueDawley rats were randomly divided into 5 groups on average: sham group (A), model group (B), benapril group (C), smalldose matrine and crataegus mixture group (D), and largedose matrine and crataegus mixture group (D). The models were established by UUO of the left side. On the 14th day after operation, the obstructed kidney was taken out. Then HE, Masson, and immunohistochemical staining of connective tissue growth factor (CTGF), Αsmooth muscle actin (ΑSMA) and collagen Ⅲ (Col Ⅲ) were conducted. After that, semiquantitative analysis was performed. Results Matrine and crataegus mixture treatment can lessen the pathological lesion and reduce the levels of CTGF, ΑSMA and Col Ⅲ. Conclusion Matrine and crataegus mixture may downregulate expressions of cytokines such as CTGF, and inhibit the activation of cell producing ECM and then the sendimentation of ECM, thus preventing renal interstitial fibrosis. 　　KEY WORDS: matrine and crataegus mixture; renal interstitial fibrosis; connective tissue