苦参碱对表皮角质形成细胞和真皮成纤维细胞P物质受体和细胞因子表达的影响.doc

苦参碱对表皮角质形成细胞和真皮成纤维细胞P物质受体和细胞因子表达的影响 （第二军医大学 长海医院 药学部，上海 200433） 摘 要： 研究对表皮角质形成细胞系HaCaT细胞和真皮成纤维细胞P物质受体表达的影响，并探讨苦参碱对P物质诱导两种细胞表达IFN-γ、IL-1β、IL-6及IL-8的影响。采用流式细胞和Western blotting苦参碱对两种皮肤细胞P物质受体表达的影响；以P物质刺激HaCaT细胞和真皮成纤维细胞，加入不同剂量的苦参碱共孵育，采用ELISA方法测定苦参碱对IFN-γ、IL-1β、IL-6及IL-8分泌的影响。苦参碱显著抑制HaCaT细胞和真皮成纤维细胞的表达；P物质刺激可以显著增加HaCaT细胞和真皮成纤维细胞IFN-γ、IL-1β、IL-8的分泌量不同浓度的苦参碱均可以抑制皮肤细胞IL-1β、IL-8的分泌，但对IFN-γ的分泌无明显影响。P物质IL-6的产生均无显著影响。 关键词： ；P物质；HaCaT细胞；真皮成纤维细胞；细胞因子Effect of matrine on the expression of substance P receptor and cytokines production in human epidermal keratinocytes and dermal fibroblasts. LIU Ji-Yong, WANG Mei, LI Feng-Qian, ZHU Quan-Gang, SUN Hua-Jun,HU Jin-Hong* (Department of Pharmacy，Changhai Hospital，Second Military Medical University，Shanghai 200433，China) ABSTRACT To investigate the effect of matrine on the expression of neurokinin 1 receptor (NK-1R) and cytokines production induced by substance P (SP) in HaCaT cells (a human epidermal keratinocyte cell line) and dermal fibroblasts. The effect of Matrine on the expression of NK-1R protein was detected by flow cytometry and Western blotting analysis.The modulation of Matrine on the production of interferon (IFN)-γ, interleukin (IL)-1β, IL-6 and IL-8 in HaCaT cells and fibroblasts was measured by ELISA. The results showed that Matrine significantly inhibited the expression of NK-1R in HaCaT cells and fibroblasts. SP induced the production of IFN-γ, IL-1β and IL-8 in both cell types. Matrine 10-100μg·mL-1 inhibited SP-induced IL-1β and IL-8 production in HaCaT cells and fibroblasts, while had no effect on the production of IFN-γ in both cells. Both SP and Matrine had no effect on the secretion of IL-6 in HaCaT cells and fibroblasts. These findings suggest that Matrine may be involved in the treatment of skin inflammation induced by SP by inhibition the expression of substance P receptor and regulation the production of IL-1β and IL-8 in epidermal keratinocyte and dermal fibroblasts. Key words: Matrine; substance P recept